肝细胞癌动态增强MRI定量分析的血供基础实验研究

目的探讨不同病理级别肝细胞癌(hepatocellular carcinoma,HCC)动态增强磁共振(magnetic resonance imaging,MRI)首次增强斜率的血供基础.方法用二乙基亚硝胺(diethylnitrosamine,DEN)长期小剂量灌喂大鼠诱发肝细胞癌,于不同时相行MRI动态增强扫描,绘制时间-信号强度曲线,计算首次增强斜率,并且在相应部位取材做病理观察及微血管密度(microvessel density,MVD)测定.结果肝细胞癌MRI动态增强扫描时间-信号强度曲线Sfirst与病理学分级有关,肿瘤恶性程度越高,其值越大.肝细胞癌MVD也与病理学分级有关,高分化肝细胞癌MVD值最小,而低分化肿瘤MVD值最大.结论MVD是导致不同病理级别肝细胞癌MRI首次增强斜率显著差异的重要原因之一.

Blood supply analysis based on quantitative dynamic contrast-enhanced MRI in hepatocellular carcinoma

Objective To analyze the status of blood supply at appearance of the first enhanced signal on dynamic contrast-enhanced MRI of hepatic cell cancer(HCC) of differentiated degree.Methods HCC was induced chronically with 1 ml of 2% DEN solution once a day in 80 rats for 14 weeks,and another 10 rats served as normal controls.All rats received dynamic contrast-enhanced MRI examination on week 16,18,20,21,22,23,24,25.The time-intensity curve was drawn and the slope rate of the first enhanced signal(S_(first)) was counted,then specimens were taken and observed of microvessel density(MVD) with light microscope.Results The differentiated degree of HCC was correlated with S_(first) and MVD.The poorer HCC differentiation,the bigger S_(first).Well differentiated HCC had low MVD,while poorly differentiated had higher MVD.Conclusion MVD is the main influence factor for that the S_(first) were significantly different between HCC of differentiated degree.