以角质形成细胞异常增生为特点的皮肤病hTR、hTERT mRNA表达

目的：探讨端粒酶RNA（hTR）／和端粒酶反转录酶（hTERT）在几种以角质形成细胞异常增生为特点的皮肤病中的表达及其与角质形成细胞增殖的关系。方法：应用原位杂交方法检测银屑病、基底细胞癌（BCC）、鳞癌（SCC）、脂溢性角化（SK）组织标本中hTR、hTERT mRNA的表达。应用免疫组织化学方法检测Ki-67抗原的表达，并对hTR、hTERT mRNA的表达与Ki-67抗原的表达行相关性分析。结果：hTR表达在基底细胞、棘细胞、颗粒层细胞层和所有有核细胞的胞浆。与细胞的增殖情况无关；其阳性细胞百分率在各组差异无统计学意义（P〉0．05）。而hTERT主要表达在癌巢和生长活跃的组织和细胞。与细胞的增殖状态有关。阳性细胞百分率高于正常皮肤（P〈0．05）。Ki-67抗原主要表达在细胞生发层和分裂旺盛的组织；其表达与hTERT的表达呈正相关（r=0．674。P〈0．01）：而与hTR（r=0．295，P〉0．05）无关。结论：hTERT mRNA与细胞增殖活性有关，在银屑病、基底细胞癌、鳞癌、脂溢性角化中的表达明显升高．可作为细胞增殖活性的分子生物学标志。

Expression of Telomerase RNA and Telomerase Catalytic Subunit Gene mRNA in Skin Diseases Characterized by Keratinocytes'''' Hyperproliferation

Objective: To study the expressions of telomerase RNA component (hTR) and telomerase catalytic subunit gene (hTERT) mRNA in some abnormal hyperproliferative skin diseases and their relationship with keratinocytes proliferation. Methods: The expressions of hTR and hTERT in the lesions of patients with psoriasis, basal cell carcinoma (BCC), squamous cell carcinoma (SCC) and seborrheic keratosis (SK) were examined with in situ hybridization. Using immunohistochemistry methods sequential sections were stained with anti-Ki-67 antibody, a proliferative marker. Results: The levels of hTR were positive (showed moderate to strong levels) in almost all basal, prickle, and granular cells of the epidermis. There was no significant difference between normal and lesional skin(P > 0.05). However, the expression of hTERT mRAN was much higher in psoriasis, BCC, SCC and SK compared with those of normal controls (P < 0.05). The expression of Ki-67 in psoriasis was much stronger than that of normal controls and statistically correlated with hTERT (r = 0.674, P < 0.01), but not with hTR (r = -0.295, P > 0.05) expression. Conclusion: Telomerase hTERT, rather than hTR, could be up-regulated in lesions from psoriasis, BCC, SCC and SK, and should be represented as a new molecular marker as Ki-67 for cell proliferation.