高效液相－串联质谱联用法测定人血浆中阿昔洛韦的浓度

目的：建立高效液相-串联质谱联用（LC-MS／MS）法测定人血浆中阿昔洛韦的浓度。方法：采用Waters公司SYMMETRY^TM C18（3．9mm×150mm，5gm）谱柱；流动相为甲醇－水（15：85）；流速为0．6mL／min。串联质谱条件：采用多反应离子监测（MRM）检测，每个样品分析时间为2min。结果：阿昔洛韦在20．0～1000．0ng／mL浓度范围内有良好的线性关系；最低检测浓度为20．0ng／mL；日内、目间RSD均〈15％；准确度RE均在±15％范围内。结论：本法简单、快速、灵敏、准确，可用于人血浆中阿昔洛韦浓度的检测。

Determination of Acyclovir in Human Plasma by LC-MS/MS Method

Objective:A sensitive and fast liquid chromatography-tandem mass spectrometry(LC-MS/MS)method was developed for the determination of acyclovir in human plasma.Methods:The column was SYMMETRY~(TM) C_(18) (3.9 mm×150 mm×5μm).The mobile phase consisted of methanol:water(15:85)and the flow rote was 0.6 mL/min.Detection was performed on a tandem mass spectrometry in multiple reaction monitoring (MRM) mode.The running time was 2 min.Results: Acyclovir has good line relation between 20.0～1 000.0 ng/mL.The limit of quantitation of acyclovir was 20.0 ng/mL.Inter-day and intra-day precision(RSD)of acyclovir was all within 15 % and the accuracy(RE)was all within±15%.Conclusion:The method is simple,fast,sensitive and accurate.It can be used for the determination of acyclovir in human plasma.