清解方雾化吸入剂的抗炎作用研究

目的考察清解方雾化吸入剂的抗炎作用及其作用机制。方法采用二甲苯致小鼠耳廓肿胀实验、冰醋酸致小鼠毛细血管通透性增加实验以及角叉菜胶致小鼠足肿胀实验,观察清解方雾化吸入剂的抗炎作用。测定肿胀足部位炎症因子NO、PGE2、IL-1β和TNF-α,探讨其抗炎作用机制。结果清解方雾化吸入剂9.2、18.4 g/kg均能显著降低小鼠耳肿胀度(P0.05、0.01),降低灌洗液吸光度值(P0.05),抑制角叉菜胶引起的足跖肿胀(P0.05),显著抑制炎症部位炎症介质NO、PGE2水平(P0.01、0.001),显著抑制炎症部位细胞因子IL-1β、TNF-α的释放(P0.001),且清解方雾化吸入剂4.6 g/kg也显著抑制IL-1β的释放(P0.01)。结论清解方雾化吸入剂具有显著的抗炎作用,作用机制可能在于降低IL-1β、TNF-α致炎细胞因子的释放,减少炎症部位NO、PGE2炎症介质的生成。

Anti-inflammatory effect of Qingjie Decoction Aerosol

Objective To investigate the anti-inflammatory effect of Qingjie Decoction Aerosol, and explore its mechanism.Methods Xylene induced ear edema experiment, glacial acetic acid increased capillary permeability experiment, and carrageenan induced foot swelling experiment were adopted to evaluate the anti-inflammatory efficacy of Qingjie Decoction Aerosol. Meanwhile, inflammatory factors NO, PGE2, IL-1β, and TNF-α in the swollen feet were determined to study its mechanism. Results Qingjie Decoction Aerosol (9.2, 18.4 g/kg) significantly reduced ear edema induced by xylene (P< 0.05, 0.001), decreased absorbance values of lavage fluid (P< 0.05), and inhibited carrageenin induced paw edema (P < 0.05). Qingjie Decoction Aerosol (9.2, 18.4 g/kg) significantly inhibited levels of inflammatory mediators NO and PGE2 (P< 0.01, 0.001), significantly inhibited realese of inflammatory cytokine IL-1β and TNF-α (P < 0.001), Qingjie Decoction Aerosol (4.6 g/kg) also significantly inhibited the release of IL-1β (P < 0.01). Conclusion Qingjie Decoction exhibited a prominent anti-inflammatory capacity, which might be mediate by IL-1β, TNF-α inhibition and NO, PGE2 reduction. Qingjie Decoction Aerosol has significant anti-inflammatory effect, which might be related to release reduction of proinflammatory cytokines IL-1β, TNF-α and generation reduction of inflammatory mediators NO and PGE2.