Anti-U1RNP antibodies in patients with localized scleroderma |
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Authors: | Kenichi Yamane H Ihn Masahide Kubo Masataka Kuwana Yoshihide Asano Norihito Yazawa Kunihiko Tamaki |
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Institution: | (1) Department of Dermatology, Faculty of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan e-mail: IN-DER@h.u-tokyo.ac.jp, Tel.: +81-3-38155411, Fax: +81-338141503, JP;(2) Division of Cellular Signaling, Institute for Advanced Medical Research, Keio University School of Medicine, Tokyo, Japan, JP |
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Abstract: | Abstract Antibodies to U1 ribonucleoproteins (RNP) have been detected in serum from patients with various autoimmune diseases. However,
the presence of anti-U1RNP antibodies in patients with localized scleroderma has not been reported. In this study, we examined
the frequency of anti-U1RNP antibodies using immunoprecipitation of U small nuclear RNAs and determined the antigen specificity
by immunoblotting. Of 70 serum samples from patients with localized scleroderma, 2 (3%) immunoprecipitated U1 small nuclear
RNA. Indirect immunofluorescence using HEp-2 cells as substrate showed coarse speckled nuclear fluorescence without nucleolar
staining in both of the samples positive for anti-U1RNP antibodies. In addition, the presence of anti-U1RNP antibodies in
each serum sample was confirmed by immunodiffusion against HeLa cell extracts. Immunoblotting analysis showed anti-70 kDa
antibodies in each serum sample. This reaction against 70 kDa protein in the patients with localized scleroderma was analogous
to that in patients with systemic sclerosis or mixed connective tissue disease. Both patients with positive serum were diagnosed
as having linear scleroderma, but neither had evidence of Raynaud’s phenomenon or sclerodactyly. These results indicate that
the presence of anti-U1RNP antibodies is one of the serological abnormalities in localized scleroderma, and that the mechanism
of induction of anti-U1RNP antibodies in patients with localized scleroderma might be similar to that in patients with systemic
sclerosis and mixed connective tissue disease.
Received: 12 February 2001 / Revised: 27 April 2001 / Accepted: 11 July 2001 |
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Keywords: | Autoantibody Immunoprecipitation Immunoblotting |
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