| 烟草甲基亚硝胺吡啶基丁酮诱发HPV—18永生化人类支气管上皮细胞恶性 … |
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| 引用本文： | 孙红琰,龚诒芬. 烟草甲基亚硝胺吡啶基丁酮诱发HPV—18永生化人类支气管上皮细胞恶性 …[J]. 卫生研究, 1999, 28(2): 95-96 |
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| 作者姓名： | 孙红琰 龚诒芬 |
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| 作者单位： | 军事医学科学院输血研究所，北京放射医学研究所，中国预防医学科学院营养与食品卫生研究所 |
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| 摘 要： | 观察了烟草特异亚硝胺ＮＮＫ诱发ＨＰＶ－１８永生化人类支气管上皮细胞（ＢＥＰ２Ｄ）恶性转化过程中细胞生物学特征的变化。５００ｍｇ／ＬＮＮＫ处理２ＢＥＰ２Ｄ细胞一天后，细胞在体外可连续传代，第５代细胞显示抗血清生长；第１５代细胞在半固体琼脂中的集落形成率（０．０３８％）为对照细胞（０．００３３％）的１１倍；第２５代细胞在裸鼠体内瘤，组织学证实为高分化鳞状细胞瘤，以上结果表明ＮＮＫ致ＢＥＰ２Ｄ细胞恶性转
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| 关 键 词： | 烟草 NNK 支气管上皮细胞 癌变 肺癌 |
Malignant transformation of HPV 18 immortalized human bronchial epithelial cells induced by tobacco specific nitrosamine NNK |
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| Sun Hongyan,Gong Yifen,Wang Quanli,Chen Junshi,et al.. Malignant transformation of HPV 18 immortalized human bronchial epithelial cells induced by tobacco specific nitrosamine NNK[J]. Journal of hygiene research, 1999, 28(2): 95-96 |
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| Authors: | Sun Hongyan Gong Yifen Wang Quanli Chen Junshi et al. |
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| Affiliation: | Institute of Transfusion, Beijing 100850, China. |
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| Abstract: | The malignant transformation of HPV-18 immortalized human bronchial epithelial cell(BEP2D) induced by tobacco-specific nitrosamine NNK and its biological characteristics were investigated. BEP2D cells treated with NNK at the concentration of 500 micrograms/ml for 24 hours could be subcultured continuously in vitro. The 15th passage cells in soft agar (0.038%) increased 11 fold compared with that of control cells (0.0033%). The 25th passage cells could grow into tumor in nude mice. The tumor was a squamous cell carcinoma in morphology confirmed by histopathological examination. The results indicated that the malignant transformation induced by NNK was a sequential process. This culture system provides a potential tool for the study of cell and molecular mechanism in the multistage carcinogenesis of human bronchial epithelial cells. |
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| Keywords: | tobacco specific nitrosamine NNK immortalized human bronchial epithelial cell malignant transformation |
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