BALB/c小鼠特异性免疫状况与中枢神经系统风疹病毒JR23株的感染

目的　探讨机体特异性免疫状况与中枢神经系统 (centralnervoussystem ,CNS)风疹病毒(rubellavirus,RV)感染的关系。方法　本研究选择BALB c小鼠 ,给予地塞米松和环磷酰胺药物后 ,经腹腔感染RVJR2 3株 ,于感染后第 2 1天检测动物的免疫功能 ,观察RV对CNS的侵袭性 ,分析两者之间的关系。结果　环磷酰胺药物组的动物细胞功能明显低于其他实验组 (P <0 0 5 ) ,地塞米松药物组、药物未干预的病毒感染组及阴性对照组之间差异无显著性 (P >0 0 5 )。CNS受染动物的细胞免疫功能明显低于CNS未受染动物 (P <0 0 0 1)。地塞米松、环磷酰胺和药物未干预动物组的CNSRV感染率分别为 6 0 % ,90 %和 5 0 %。各组感染动物RV特异性抗体生成差异无显著性 (P >0 0 5 )。结论　在机体尚未产生特异性抗体前 ,RV对CNS的感染与机体的细胞免疫状况有关

Immune status of BALB/c mice and rubella virus JR23 strain infection of central nervous system

Objective To investigate the relationship between immune status and rubella virus (RV) infection of central nervous system (CNS). Methods BALB/c mice were given dexamethaxone and cytoxan before RV JR23 strain infection. Immune functions and RV invasion to CNS were assayed at 21 days postinfection via abdominal cavity and their relationship was analyzed.Results T cell functions of cytoxan group were obviously worse than those of other groups (%P%<0.05) by MTT method. Infection rates of dexamethaxone and cytoxan and the group without any intervention were 60%,90% and 50%(P>0.05),respectively. Cellular immune functions of the mice with CNS infection were obviously worse than those of the mice without CNS infection (%P%<0.001). Specific antibodies (Ab) were assayed in all groups with ELISA and the results showed that there were no significant differences among groups (P>0.05), neither between the groups with and without CNS infections.Conclusion RV infection of CNS may relate to cellular immune status before specific antibody was produced in the body.