阿司匹林对人胃癌细胞株的作用及机制探讨

目的 观察阿司匹林对人胃癌细胞株SGC7901生长及人胃癌裸鼠移植瘤生长的作用,并初步探讨其作用机制。方法 采用MTT法及流式细胞术检测不同浓度阿司匹林对胃癌细胞增殖及细胞周期的影响;Westem blot法检测阿司匹林对胃癌细胞COX-2、VEGF表达的影响;建立人胃癌裸鼠移植瘤模型,给予阿司匹林20天,观察肿瘤大小,免疫组化检测阿司匹林对肿瘤组织中COX-2、VEGF表达及MVD的影响。结果 阿司匹林对胃癌细胞的增殖具有抑制作用,且呈一定的时间、剂量依赖性;细胞周期分析表明阿司匹枳主要使细胞阻滞在G0/G1期;western blot检测表明阿司匹林能降低胃癌细胞COX-2、VEGF蛋白的表达;阿司匹林对裸鼠移植瘤的生长有抑制作用,免疫组化显示阿司匹林能降低移植瘤组织中(COX-2、VEGF的表达及MVD。结论 阿司匹林对胃癌细胞及裸鼠移植瘤均具有一定的抑制作用,其机制可能是通过对COX-2和VEGF等血管生成相关因子的抑制起作用。

Inhibitory effects of Aspirin on gastric cancer cells in vitro and in vivo

Objective To investigate the effects of aspirin both on human gastric cancer cell line SGC7901 and its transplanted tumors on nude mice, trying to explore the possible mechanism involved.Methods MTT and, flow cytometry (FCM) method were used to study the effects of aspirin on cell growth. Western blot was used to investigate the influence of aspirin on the expression of COX-2 and VEGF protein in the cell line. Nude mice bearing gastric cancer xenografts were treated with aspirin 10mg/kg for 20 days, and the tumor volume was measured. Immunohistochemsitry was used to investigate aspirin's influence on the expression of COX-2 , VEGF and MVD in tumor tissues. Results There was a time-and dose-dependent inhibition of cell proliferation by aspirin on cultured SGC7901 cells. Cell cycle analysis showed that the cells were mainly blocked in G0/G1 phase. Aspirin can inhibit the expression of COX-2 and VEGF. In nude mice bearing human gastric cancer xenografts, aspirin also showed a suppressive effect on tumor growth and can reduce the expression of COX-2 , VEGF and MVD in tumor tissues. Conclusions Aspirin has inhibitory effects both on SGC7901 cells and its transplanted tumors in nude mice. Antiangiogenesis may be one of the mechanisms by which aspirin exerts its tumor chemopreventive and therapeutic effects.