光学法计数在低值血小板检测中的研究应用

目的：评价光学法计数在低值血小板检测中的研究应用。方法：200例低值血小板患者血标本，在血液分析仪上分别进行电阻抗法和光学法血小板计数，同时进行镜检法计数。结果：以镜检法为参考方法，光学法的相关系数为0．994。电阻抗法的相关系数0．921。在小红细胞组、红细胞碎片组和大血小板组，电阻抗法和镜检法计数结果差异有统计学意义（P〈0．05），光学法和镜检法计数结果差异无统计学意义（P〉0．05）。结论：光学法计数可以满足低值血小板检测准确性的要求，值得临床实验室推广应用。

Application of optical platelet count in low platelet samples

Objective：To evaluate the application of optical method in low platelet counts. Method：200 sampies with low platelet counts were investigated by resistance rnethod（PLT-I） and optical method（PLT-O） on automated hematology analyzer and by microscopic method which was regarded as reference method. Result：Compared with microscopic method, the correlative coefficients of the PLT-O and PLT-I were 0. 994 and 0. 921, respectively. Small RBC and RBC fragments as well as large PLT particles interfered with PLT-I and the results were statis tically different from those of microscopic method（ P〈0. 05）. But there were no statistical differences between PLT-O and microscopic method （ P 〉0. 05）. Conclusion： PLT O was able to meet the requirement of accuracy for low platelet counts. It should be used in clinical laboratories when low platelet counts happened.