GDF11在体外扩增CD8+记忆干性T细胞的作用

 目的: 探讨生长分化因子11(GDF11)在体外诱导扩增具有干细胞特性的记忆性T细胞(memory stem T cells,Tscm)中的作用,从而进一步提高免疫过继治疗的疗效。方法: 通过密度梯度离心的方法分离健康人的外周血单个核细胞,然后用磁珠分选获得CD8⁺T细胞;随后将所得的细胞分为实验组和对照组,实验组中加入等体积、不同浓度的GDF11,对照组中加入等体积的PBS缓冲液,最后分别在不同时点通过流式细胞术检测2组细胞中Tscm的扩增情况。结果: 在CD8⁺T细胞的体外扩增实验中,GDF11可以显著扩增CD8⁺T细胞;在CD8⁺T细胞的体外培养实验中,GDF11可以显著提高Tscm在CD8⁺T细胞中的比例。结论: GDF11可以有效地在体外扩增CD8⁺Tscm,为提高免疫过继治疗的效率提供了新的方法。

Effect of GDF11 on expansion of CD8+ memory stem T cells

AIM: To investigate the effect of growth differentiation factor 11(GDF11) on the expansion of CD8⁺ memory stem T cells(Tscm) and to further improve the effect of adoptive immunotherapy. METHODS: Healthy human peripheral blood mononuclear cells(PBMCs) were isolated by density gradient centrifugation at first. Among the isolated PBMCs, CD8⁺ T cells were further purified with MACS microbeads. The CD8⁺ T cells were then randomly divided into experimental groups and control group. The same volume of different concentrations of GDF11 were added into the experimental groups, and the same volume of PBS solution was added into the control group. Finally, the expansion of Tscm in experimental groups and control group was measured by flow cytometry at several time points. RESULTS: GDF11 significantly increased the number of Tscm in CD8⁺ T cells in vitro expansion and also dramatically increased the ratio of Tscm in CD8⁺ T cells. Furthermore, 400μg/L GDF11 treatment for 3 weeks was the optimal condition to induce CD8⁺ Tscm. CONCLUSION: GDF11 effectively increases the number and ratio of Tscm in the CD8⁺ T cells in cell culture growth, thereby creating a new strategy to further improve the efficiency of adoptive immunotherapy.