川芎嗪通过抗炎作用减轻2型糖尿病大鼠心肌病变

目的:观察川芎嗪对抗糖尿病引起的大鼠心肌细胞损伤的作用。方法:雄性Wistar大鼠高糖高脂饲料喂养4周后空腹自尾静脉注射STZ制造2型糖尿病模型,观察不同给药剂量川芎嗪对糖尿病大鼠体重、血糖、心脏组织形态以及心肌组织中IL-1β、IL-6、TNF-α、IKKβ和NF-κB蛋白水平的影响。结果:高剂量川芎嗪可缓解糖尿病大鼠的体重减轻和血糖升高,并且可以减少促炎因子IL-1β、IL-6和TNF-α的表达,降低IKKβ和NF-κB的蛋白水平。结论:川芎嗪通过拮抗炎症通路能减轻糖尿病引起的大鼠心肌细胞损伤。

Effect of ligustrazine on cardiac inflammation in rats with type 2 diabetes mellitus

AIM: To study the effect of ligustrazine on the cardiacmyocyte lesion in rats with type 2 diabetes mellitus. METHODS: Male Wistar rats were injected with STZ via tail vein under high-glucose and high-fat feeding for 4 weeks to establish the animal model of type 2 diabetes mellitus. Ligustrazine at different doses was used to treat the diabetic rats. The body weight, blood glucose and the morphology of heart tissues were observed. The myocardial levels of IL-1β, IL-6 and TNF-α were detected by ELISA, and the protein expression of IKKβ and NF-κB in the myocardium was determined by Westeren blotting. RESULTS: Ligustrazine at high dose alleviated the body weight reduction and blood glucose elevation cause by diabetes, and reduced pro-inflammatory factors IL-1β, TNF-α and IL-6. Moreover, the protein expression of IKKβ and NF-κB was significant decreased by ligustrazine. CONCLUSION: Ligustrazine inhibits the myocardial inflammation caused by diabetes through anti-inflammatory pathway.