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miR-193b体外协同增强多柔比星对乳腺癌细胞的抗肿瘤效应
引用本文:应晓,王再红,王振华. miR-193b体外协同增强多柔比星对乳腺癌细胞的抗肿瘤效应[J]. 中国病理生理杂志, 2015, 31(9): 1584-1588. DOI: 10.3969/j.issn.1000-4718.2015.09.009
作者姓名:应晓  王再红  王振华
作者单位:衢州市中医医院检验科, 浙江 衢州 324002
摘    要:目的:研究微小RNA(microRNA,miR)-193b是否能增强多柔比星对乳腺癌细胞的杀伤效力及机制。方法:用real-time PCR方法检测乳腺癌患者及健康对照者血浆中的miR-193b表达水平。MTT法检测miR-193b联合多柔比星对乳腺癌细胞系MDA-MB-231的杀伤效力。利用生物信息学、real-time PCR及Western blot方法验证miR-193b是否调节乳腺癌细胞Mcl-1的表达。构建Mcl-1真核表达载体,MTT法检测Mcl-1表达载体转染对miR-193b联合多柔比星治疗乳腺癌疗效的影响。结果:乳腺癌患者血浆中miR-193b表达水平显著低于对照组。miR-193b联合多柔比星治疗组对MDA-MB-231细胞的杀伤效力显著高于多柔比星单治疗组。miR-193b转染后,MDA-MB-231细胞Mcl-1的mRNA及蛋白表达水平均下降。miR-193b联合多柔比星在Mcl-1表达载体转染后对MDA-MB-231细胞的杀伤活性显著低于未转染Mcl-1表达载体的miR-193b联合多柔比星组。结论:miR-193b通过靶向于Mcl-1增强多柔比星对乳腺癌细胞的杀伤效力。

关 键 词:微小RNA-193b  Mcl-1  乳腺癌  MDA-MB-231细胞  多柔比星  
收稿时间:2015-04-23

miR-193b enhances cytotoxicity of doxorubicin by targeting Mcl-1 in breast cancer
YING Xiao,WANG Zai-hong,WANG Zhen-hua. miR-193b enhances cytotoxicity of doxorubicin by targeting Mcl-1 in breast cancer[J]. Chinese Journal of Pathophysiology, 2015, 31(9): 1584-1588. DOI: 10.3969/j.issn.1000-4718.2015.09.009
Authors:YING Xiao  WANG Zai-hong  WANG Zhen-hua
Affiliation:Clinical Laboratory, Quzhou Municipal Hospital of Traditional Chinese Medicine, Quzhou 324002, China
Abstract:AIM: To investigate the effect of microRNA(miR)-193b on doxorubicin therapy in breast cancer in vitro.METHODS: miR-193b level in plasma was detected by real-time PCR in the patients with breast cancer or the healthy controls. MTT assay was performed to measure the inhibitory effect of miR-193b plus doxorubicin on the growth of MDA-MB-231 cells. Bioinformatics, real-time PCR and Western blot were performed to determine whether the expression of Mcl-1 was regulated by miR-193b. Mcl-1 expression vector was constructed, and the role of Mcl-1 vector toward miR-193b plus doxorubicin-induced cytotoxicity in MDA-MB-231 cells was observed by MTT assay.RESULTS: Down-regulation of miR-193b was found in breast cancer patients. The miR-193b plus doxorubicin group showed a higher growth inhibition than cisplation group in MDA-MB-231 cells. The expression of Mcl-1 at both mRNA and protein levels was down-regulated after miR-193b transfection. The growth inhibition of MDA-MB-231 cells treated with miR-193b plus doxorubicin was significantly decreased after the transfection of Mcl-1 expression vector.CONCLUSION: miR-193b sensitizes doxorubicin-induced cytotoxicity by targeting Mcl-1 in breast cancer.
Keywords:MicroRNA-193b  Mcl-1  Breast cancer  MDA-MB-231 cells  Doxorubicin
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