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| 白杨素对大鼠主动脉舒张作用的影响 | |
| 引用本文： | 田晴晴,罗乐,张夏丽,陈敏,李洁,高雪梅,张明升,张轩萍. 白杨素对大鼠主动脉舒张作用的影响[J]. 中国病理生理杂志, 2016, 32(4): 618-622. DOI: 10.3969/j.issn.1000-4718.2016.04.007 |
| 作者姓名： | 田晴晴 罗乐 张夏丽 陈敏 李洁 高雪梅 张明升 张轩萍 |
| 作者单位： | 1. 山西医科大学药理教研室, 山西 太原 030001; 2. 山西职工医学院生理学教研室, 山西 太原 030001 |
| 基金项目： | 山西省自然科学基金资助项目(No.2011011034-3);山西省卫生科研课题(No.2015109) |
| 摘 要： | 目的: 研究白杨素(chrysin)对离体大鼠主动脉肌源性反应的影响,并探讨其作用机制。方法: 分离SD大鼠主动脉,采用离体血管环灌流装置观察chrysin对血管环的基础张力及对60 mmol/L KCl预收缩血管的舒张作用,并结合不同抑制剂处理,探讨其作用于血管环的可能机制。结果: Chrysin(10-6 mol/L、3×10-6 mol/L、10-5 mol/L、3×10-5 mol/L和10-4 mol/L)对基础状态血管无明显影响,但对60 mmol/L KCl预收缩的血管环具有浓度依赖性舒张作用,并且去内皮组舒张作用弱于内皮完整组(P<0.05)。NOS抑制剂L-NAME(10-4 mol/L)处理血管后,chrysin的舒张血管作用部分被抑制(P<0.05),COX抑制剂吲哚美辛(10-5 mol/L)处理血管后无明显抑制作用。钾通道阻滞剂4-氨基吡啶(10-3 mol/L)、氯化钡(10-4 mol/L)、格列苯脲(10-5 mol/L)和四乙胺(10-3 mol/L)预孵后,chrysin舒张血管作用均被部分抑制(P<0.05)。Chrysin(10-6 mol/L、10-5 mol/L和10-4 mol/L)可浓度依赖性抑制2.5 mmol/L CaCl2引起的主动脉收缩。结论: Chrysin能够浓度依赖性舒张大鼠主动脉,其作用机制可能与促进一氧化氮释放、激活4种K+通道及减少细胞内钙离子浓度有关。 |
| 关 键 词： | 白杨素 主动脉环 一氧化氮合酶 环氧合酶 钾通道 钙 |
| 收稿时间： | 2015-10-20 |
Effect of chrysin on relaxation of isolated rat aortic ring |
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| TIAN Qing-qing,LUO Le,ZHANG Xia-li,CHEN Min,LI Jie,GAO Xue-mei,ZHANG Ming-sheng,ZHANG Xuan-ping. Effect of chrysin on relaxation of isolated rat aortic ring[J]. Chinese Journal of Pathophysiology, 2016, 32(4): 618-622. DOI: 10.3969/j.issn.1000-4718.2016.04.007 | |
| Authors: | TIAN Qing-qing LUO Le ZHANG Xia-li CHEN Min LI Jie GAO Xue-mei ZHANG Ming-sheng ZHANG Xuan-ping |
| Affiliation: | 1. Department of Pharmacology, Shanxi Medical University, Taiyuan 030001, China; 2. Department of Physiology, Shanxi Medical College for Conti-nuing Education, Taiyuan 030001, China |
| Abstract: | AIM: To determine the effect of chrysin on the relaxation of isolated rat aortic rings and its mechanisms. METHODS: On ex vivo aortic ring perfusion device, the influence of chrysin on isolated aortic ring at basic condition or contraction of the aorta induced by KCl(60 mmol/L) was observed, and the effects of chrysin on the blood vessel reaction induced by various drugs were recorded. RESULTS: Chrysin(10-6 mol/L, 3×10-6 mol/L, 10-5 mol/L, 3×10-5 mol/L and 10-4 mol/L) had no effect on isolated aortic ring at basic condition. Chrysin had stronger vasodilatation effect in intact group than that in non-intact group(P<0.05). Treatment of the arterial rings with eNOS inhibitor L-NAME, but not with indomethecin or endothelium denudation, obviously affected the relaxant effects of chrysin(P<0.05). When the contractions were induced by KCl, 4-aminopyridine(10-3 mol/L), BaCl2(10-4 mol/L), glibenclamide(10-5 mol/L), and tetraethylammonium(10-3 mol/L) weakened chrysin-induced diastolic effect(P<0.05). Chrysin significantly inhibited contracting effect of KCl on aorta rings in 2.5 mmol/L Ca2+ medium(P<0.05). CONCLUSION: Chrysin exerts vasodilating effect on rat isolated aorta rings in a dose-dependent manner. The mechanism might be related to promoting NO release, activating K+ channels and decreasing the concentration of cytoplasmic Ca2+. |
| Keywords: | Chrysin Aorta rings Nitric oxide synthase Cyclooxygenase Potassium channels Calcium |
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