沉默URG11基因对前列腺癌LNCaP细胞增殖、迁移与侵袭的影响

 目的: 观察上调基因11(up-regulated gene 11,URG11)在前列腺癌细胞系中的表达及降低URG11表达对人前列腺癌LNCaP细胞增殖和侵袭能力的影响。方法: 用real-time PCR和Western blot检测前列腺癌细胞系和正常前列腺上皮细胞系中URG11 mRNA和蛋白水平;设计针对URG11基因的siRNA靶序列,转染LNCaP细胞,采用流式细胞仪检测细胞周期和凋亡,MTS测定LNCaP细胞增殖能力,划痕和侵袭实验评价LNCaP细胞迁移及侵袭能力。结果: 在LNCaP、DU145、PC3前列腺癌细胞系和RWPE-1正常前列腺上皮细胞系中URG11 mRNA和蛋白表达水平存在显著差异,在前列腺癌细胞中URG11 mRNA和蛋白表达水平明显升高(P<0.05)。与对照组相比,转染URG11 siRNA的LNCaP细胞增殖停滞在G₁/S期并诱导前列腺癌细胞凋亡,转染组的细胞凋亡率为8.79%±0.12%,而且LNCaP肿瘤细胞的迁移及侵袭能力明显下降(P<0.05)。结论: URG11在前列腺癌系中高表达。通过RNAi沉默URG11基因能明显抑制LNCaP细胞增殖和侵袭能力,并诱导细胞凋亡。

Effect of URG11 gene silencing on proliferation and invasion abilities of human prostate cancer cells

AIM: To investigate the expression of up-regulated gene 11(URG11) in prostate cancer cell line and the effect of URG11 siNRA on the proliferation and invasion of human prostate cancer LNCaP cells. METHODS: The mRNA and protein levels of URG11 in prostate cancer cell lines and normal prostate epithelial cell line were evaluated by real-time PCR and Western blot. LNCaP cells were transfected with designed siRNA using the liposome method. The proliferation, apoptosis, migration and invasion abilities of the LNCaP cells were evaluated by MTS assay, flow cytometry, wound-healing assay and Transwell assay. RESULTS: The expression of URG11 at mRNA and protein levels in the DU145, PC3, LNCaP cell lines was significantly higher than that in RWPE-1 cell line. Compared with the control group, the proliferation of LNCaP cells with URG11 siRNA was stagnant in G₁/S phase and induced apoptosis. The proliferation of LNCaP cells at 0 h, 24 h, 48 h and 72 h was inhibited after URG11 expression was down-regulated(P<0.05). Transwell assay showed that migration(P<0.05) and invasion(P<0.05) were also inhibited. CONCLUSION: URG11 is highly expressed in prostate cancer. Silencing of URG11 significantly inhibits the proliferation and invasion and induces apoptosis of LNCaP cells.