活血定眩胶囊含药血清减轻小鼠脑微血管内皮细胞bEnd.3缺氧损伤

目的:研究活血定眩胶囊含药血清对抗缺氧所致小鼠脑微血管内皮细胞bEnd.3损伤的作用。方法:将30只大鼠随机分为空白对照组15只和活血定眩胶囊组15只,采集2组大鼠血清。将bEnd.3细胞分为正常组、缺氧模型组和活血定眩胶囊血清组,给药后,bEnd.3细胞缺氧6 h。显微镜下观察细胞形态,流式细胞术检测细胞凋亡率及细胞周期,按试剂盒方法检测细胞上清液超氧化物歧化酶(SOD)活性和丙二醛(MDA)的含量。结果:活血定眩胶囊含药血清可显著对抗缺氧造成的损伤,明显改善bEnd.3细胞的形态,使缺氧所致细胞凋亡数明显减少,有效抑制缺氧诱导的bEnd.3细胞发生G_1/S期阻滞,抑制MDA生成,增强SOD活性。结论:活血定眩胶囊对缺氧所致bEnd.3细胞的损伤具有明显的对抗作用,其机制与增强细胞抗氧化能力、抑制细胞凋亡有关。

Protective effect of Huoxue-Dingxuan capsule-medicated serum on mouse brain microvascular endothelial cell line bEnd.3 with hypoxic injury

AIM: To investigate the protective effect of Huoxue-Dingxuan capsule-medicated serum on mouse brain microvascular endothelial cell line bEnd.3 with hypoxic injury. METHODS: The Wistar rats were randomly divided into control group and Huoxue-Dingxuan capsule group. The serum was collected at the 7th day after drug administration. The bEnd.3 cells were divided into normal group, hypoxia serum model group and Huoxue-Dingxuan capsule serum group. After administration of the medicated serum, bEnd.3 cell were treated with hypoxia for 6 h. The cell morphology was observed under microscope, the apoptosis rate and cell cycle were detected by flow cytometry, and the activity of superoxide dismutase (SOD) and the content of malondialdehyde (MDA) were detected by ELISA.RESULTS: Compared with other groups, Huoxue-Dingxuan capsule-medicated serum significantly resisted the injury caused by hypoxia, obviously improved the morphology of bEnd.3 cells, significantly reduced the number of apoptotic cells induced by hypoxia, and effectively inhibited the occurrence of hypoxia-induced G₁/S phase arrest in the bEnd.3 cells. At the same time, the medicated serum inhibited MDA production, and increased SOD activity.CONCLUSION: Huoxue-Dingxuan capsule attenuates hypoxia-induced bEnd.3 cell damage by enhancing the antioxidant capacity of cells and inhibiting cell apoptosis.