橙皮苷对慢性支气管炎大鼠炎性因子表达的影响

目的:观察橙皮苷对慢性支气管炎大鼠模型的炎性因子表达变化的影响。方法:将大鼠随机分为3组:对照组、模型组和橙皮苷给药组。采用熏烟法建立慢性支气管炎大鼠模型,采用HE染色检测支气管肺组织的病理学情况;ELISA法检测支气管肺泡灌洗液中肿瘤坏死因子α(TNF-α)、白细胞介素6(IL-6)和白细胞介素10(IL-10)含量;Western blot法检测肺组织中ICAM-1和VCAM-1的蛋白表达水平。结果:与模型组相比,橙皮苷给药组的支气管肺组织的炎性浸润减少,肺泡结构的完整性增加,支气管肺泡灌洗液中TNF-α和IL-6的含量显著下降(P0.05),IL-10含量无显著变化;肺组织中ICAM-1和VCAM-1的蛋白表达水平显著下降(P0.05)。结论:橙皮苷具有降低慢性支气管炎中炎性因子TNF-α和IL-6含量的作用,可能与调节ICAM-1和VCAM-1的蛋白表达有关。

Effects of hesperidin on inflammatory cytokine levels in chronic bronchitic rats

AIM: To observe the effects of hesperidin on the inflammatory cytokine levels in chronic bronchitic rats.METHODS: The rats were randomly divided into control group, model group and hesperidin treatment group. The rat model of chronic bronchitis was established by smoking. The pathological changes of the bronchial and lung tissues were observed by HE staining. The levels of TNF-α, IL-6 and IL-10 in the bronchoalveolar lavage fluid were analyzed by ELISA. The protein expression levels of ICAM-1 and VCAM-1 were detected by Western blot.RESULTS: Compared with model group, hesperidin treatment significantly reduced inflammatory infiltration in the bronchial and lung tissues, improved the integrity of the alveolar structure, and decreased the levels of TNF-α and IL-6 in the bronchoalveolar lavage fluid, but had no effect on the IL-10 level. Moreover, the protein expression of ICAM-1 and VCAM-1 were dramatically inhibited after hesperidin treatment.CONCLUSION: Hesperidin decreases the levels of TNF-α and IL-6 in the bronchoalveolar lavage fluid in the rats with chronic bronchitis, which may be associated with the inhibition of ICAM-1 and VCAM-1 expression.