木犀草素对人肝癌HepG2细胞株侵袭、迁移和黏附能力的影响

目的:探讨木犀草素对人肝癌HepG2细胞株侵袭、迁移和黏附能力的影响及其作用机制。方法:采用不同浓度木犀草素处理体外培养的人肝癌HepG2细胞株,Transwell实验检测细胞的侵袭能力,划痕实验检测细胞的迁移能力,黏附实验评价细胞的黏附能力,Western blot检测E-钙黏蛋白(E-cadherin)、N-钙黏蛋白(N-cadherin)、波形蛋白(vimentin)和Snai1蛋白的表达。结果:木犀草素可明显降低肝癌HepG2细胞的体外侵袭、迁移及黏附能力(P0.01),且在一定浓度范围内呈明显量效关系。木犀草素处理肝癌细胞后,上皮细胞标志蛋白E-cadherin表达明显上调,间质细胞标志蛋白N-cadherin和vimentin以及转录因子Snai1表达均明显下调,木犀草素对以上蛋白的调节呈明显浓度依赖性。结论:木犀草素体外具有抑制肝癌HepG2细胞株侵袭、迁移和黏附的作用,其作用机制可能与调控上皮-间质转化有关。

Effects of luteolin on invasion,migration and adhesion of human hepatocellular carcinoma HepG2 cells

AIM: To investigate the effects of luteolin on invasion, migration and adhesion of human hepatocelluar carcinoma HepG2 cells.METHODS: HepG2 cells were cultured and treated with luteolin at 10, 20 and 40 μmol/L respectively. The invasion capability was examined by cell invasion assay. The migration ability was examined by wound healing assay. The adhesion capability was measured by adhesion assay. The protein levels of E-cadherin, N-cadherin, vimentin and Snai1 were determined by Western blot analysis.RESULTS: Luteolin inhibited the invasion, migration and adhesion ability of HepG2 cells in vitro in a dose-dependent manner. After treatment with luteolin, the expression of E-cadherin was increased significantly and the expression of N-cadherin, vimentin and Snai1 were decreased significantly.CONCLUSION: Luteolin inhibits the invasion, migration and adhesion ability of human hepatocelluar carcinoma HepG2 cells. The mechanism may be related to the regulatory effects of luteolin on epithelial-mesenchymal transition.