钙库操纵性钙通道在人循环纤维细胞中的表达及功能

 目的: 研究钙库操纵性钙通道(store-operated calcium channels,SOCC)相关功能蛋白ORAI1-3和STIM1-2在人循环纤维细胞(circulating fibrocytes)中的表达及SOCC对人循环纤维细胞分化的影响。方法: 采集健康人外周静脉血,分离出单个核细胞,体外培养分化为循环纤维细胞。采用RT-PCR和real-time PCR检测循环纤维细胞中ORAI1-3及STIM1-2的mRNA表达情况,并检测SOCC抑制剂对循环纤维细胞分化的影响。结果: Real-time PCR检测结果显示ORAI1-3和STIM1-2 mRNA在循环纤维细胞中有较高的表达水平,并且SOCC抑制剂SKF-96365对循环纤维细胞分化具有明显的抑制作用。结论: SOCC表达于循环纤维细胞中,并且影响循环纤维细胞的分化。

Expression and function of store-operated calcium channels in human circulating fibrocytes

AIM: To investigate the expression and function of store-operated calcium channels(SOCC) in human circulating fibrocytes.METHODS: Peripheral blood mononuclear cells(PBMCs) were isolated and cultured in serum-free media. After 7 d, the PBMCs differentiated into fibrocytes. RT-PCR and real-time PCR were performed to determine the mRNA expression of ORAI1-3 and STIM1-2 in the fibrocytes. SOCC inhibitor SKF-96365 was used to elucidate the role of SOCC in the differentiation of fibrocytes. RESULTS: The results of real-time PCR showed that the mRNA expression of ORAI1-3 and STIM1-2 was positive in cultured fibrocytes. SKF-96365(10μmol/L) significantly inhibited the differentiation of fibrocytes.CONCLUSION: SOCC-related proteins ORAI1-3 and STIM1-2 are abundantly expressed in the fibrocytes, and may play an important role in the differentiation of these cells.