| 高效液相色谱法测定小儿解感片中黄芩苷含量 |
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| 引用本文： | 祁雪,汪湄,李凤丽. 高效液相色谱法测定小儿解感片中黄芩苷含量[J]. 中国卫生工程学, 2008, 7(5): 303-304 |
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| 作者姓名： | 祁雪 汪湄 李凤丽 |
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| 作者单位： | 1. 吉林省人民医院,吉林,长春,130021
2. 北华大学药学院 |
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| 摘 要： | 目的建立测定小儿解感片中黄芩苷含量的高效液相色谱法。方法采用Agilent C18（5μm，250mm×4．6mm）柱，以甲醇-水-磷酸（43：57：0．2）为流动相，流速1．0ml／min，检测波长278nm，柱温35℃。结果黄芩苷在0．06～0．54μg范围内呈良好的线性关系（r=0．99998），平均回收率为99．1％。结论该方法简单，重复性好，适合用于小儿解感片的质量控制。
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| 关 键 词： | 小儿解感片 黄芩苷 高效液相色谱法 |
Determination of Baicalin in Jiegan Tablet Treating Children with a Cold by High Performance Liquid Chromatography |
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| QI Xue,WANG Mei,LI Feng-li. Determination of Baicalin in Jiegan Tablet Treating Children with a Cold by High Performance Liquid Chromatography[J]. Chinese Journal of Public Health Engineering, 2008, 7(5): 303-304 |
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| Authors: | QI Xue WANG Mei LI Feng-li |
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| Affiliation: | QI Xue , WANG Mei , LI Feng -li.( The People＇s Hospital of Jilin Province , Changchun Jilin 130021 , China ) |
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| Abstract: | Objective To establish a method for determination of baicalin in Jiegan tablet treating cold in children by high performance liquid chromatography. Methods The radix scutellariae in the content of the baicalin was drawn up. Agilent C18 column （250 mm ×4.6 mm,5 μm） was used,the mobile phase consisted of methyl alcohol -water - phosphoric acid（43： 57： 0. 2） at a flow rate of 1.0 ml/min. The wave length was set at 278 nm and the column temperature was maintained at 35 ℃. Results The baicalin presented the good linear range of 0. 06 - 0. 54 μg, r = 0. 999 98, the average recovery rate was 99. 1%. Conclusion The method is simple, result is good reproducible, and is suitable for the quantity control of Jiegan tablet. |
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| Keywords: | Jiegan table treating children with a cold Baicalin HPLC |
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