| 粮食、血中痕量硒蛋氨酸的溴化氰——荧光测定法 |
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| 引用本文： | 杨晓光,周瑞华.粮食、血中痕量硒蛋氨酸的溴化氰——荧光测定法[J].卫生研究,1997,26(1):56-59. |
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| 作者姓名： | 杨晓光 周瑞华 |
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| 作者单位： | 中国预防医学科学院营养与食品卫生研究所 |
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| 摘 要： | 本法是利用溴化氰与硒蛋氨酸定量生成硒氰酸甲酯（ＣＨ３ＳｅＣＮ），ＣＨ３ＳｅＣＮ经氯仿萃取后用混合酸消化为Ｓｅ（ＩＶ），再用２，３－二氨基萘测定苤硒脑的荧光值推算硒蛋氨酸含量。本法硒蛋氨酸最低检出限为３ｎｇ／ｇ。用硒含量１０～５００ｎｇ的硒蛋氨酸标准进行测定，其回收率为９１．８％～９７．６％，相对偏差为１．９％～６．３％。样品回收率为９２．３％～９６．７％，相对偏差为２．７％～９．０％。硒胱氨酸、硒半胱氨酸、亚硒酸钠和蛋氨酸对测定无干扰。本法的精密度和准确度均较好。
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| 关 键 词： | 硒蛋氨酸 粮食 血 |
Method of Cyanogen Bromide Fluorimetry Determination of Trace Amount of Selenomethionine in Grain and Blood |
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| Yang Xiaoguang,Zhou Ruihua,Ha Pengcheng,Tian Yuan,et al..Method of Cyanogen Bromide Fluorimetry Determination of Trace Amount of Selenomethionine in Grain and Blood[J].Journal of Hygiene Research,1997,26(1):56-59. |
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| Authors: | Yang Xiaoguang Zhou Ruihua Ha Pengcheng Tian Yuan |
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| Institution: | Institute of Nutrition & Food Hygiene, CAPM, Beijing 100050, China. |
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| Abstract: | Selenomethionine (SeMet) reacted with cyanogen bromide (BrCN) quantitatively forms CH3SeCN. After extracted with CHCl3, the Se of CH3SeCN is acid-digested to Se(IV). Then 2,3-diaminonaphthalene is used to determine the fluorescent Se value of 4,5-benzopiaselenol. The determination limit of this method was 3 ng/g SeMet. The accuracy of 10-500 ng Se in SeMet standard was 91.8%-97.6%. RSD was 1.9%-6.3%. Recoveries for grain and blood were 92.3%-96.7%. RSD was 2.7%-5.1%. The RSD for samples was 2.7%-9.0%. Selenocystine, selenocystiene selenite and methionine did not interfere with the determination. |
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| Keywords: | selenomethionine grain blood |
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