| NADPH-细胞色素P-450还原酶的制备及在外来化合物代谢中的作用 |
| |
| 引用本文： | 王莉娥. NADPH-细胞色素P-450还原酶的制备及在外来化合物代谢中的作用[J]. 卫生研究, 1997, 26(4): 217-220 |
| |
| 作者姓名： | 王莉娥 |
| |
| 作者单位： | 中国预防医学科学院劳动卫生与职业病研究所 |
| |
| 摘 要： | ＮＡＤＰＨ－细胞色素Ｐ－４５０还原酶是肝微粒体酶系的主要组成成分。本文报道了一种简单经济制备还原酶的方法，并通过ＮＡＤＰＨ的氧化证明还原酶及细胞色素Ｐ－４５０２Ｂ１重组酶系能代谢苯、二氯乙烯和石棉。但代谢过程中的中间产物未能引起质粒ＤＮＡ电泳图谱的变化。
|
| 关 键 词： | NADPH－细胞色素P-450还原酶 制备 外来化合物 代谢 |
Preparation of NADPH Cytochrome P 450 Reductase and the Role in Metabolism of Xenobiotics |
| |
| Wang Li e, Yang Mingxue, Xie Guangyun. Preparation of NADPH Cytochrome P 450 Reductase and the Role in Metabolism of Xenobiotics[J]. Journal of hygiene research, 1997, 26(4): 217-220 |
| |
| Authors: | Wang Li e Yang Mingxue Xie Guangyun |
| |
| Affiliation: | Institute of Occupational Medicine, Chinese Academy of Preventive Medicine, Beijing, China. |
| |
| Abstract: | NADPH Cytochrome P 450 Reductase is the major part of MFO (mixed function oxidase) system of liver. A simple and economic method of preparation of reductase was introduced in this paper and it was certified that xenobiotics benzene, bichloroethylene, and asbestos can be oxidized by reductase and reconstituted cytochrome P 450 2B 1. But the metabolites of the 3 compounds can not cause the changes of plasmid PBR322 DNA. |
| |
| Keywords: | NADPH cytochrome P 450 reductase preparation xenobiotics metabolism |
| 本文献已被 CNKI 维普 等数据库收录！ |
