大鼠骨矿物质含量与组织中钙水平的相关性研究

用ＳＤ１０００型单光子骨矿物测定仪研究了９８只１５周龄Ｗｉｓｔａｒ大鼠的股骨矿物质含量（ＢＭＣ）和骨密度（ＢＭＤ，ＢＭＣ／ＢＷ）与组织和血中钙含量的相关性。结果显示：雌、雄动物的身长、体重、骨宽度（ＢＷ）、ＢＭＣ、ＢＭＣ／体重、ＢＭＤ／体重及ＢＭＤ／身长均有显著性差异，活体ＢＭＤ与动物的钙总摄入量、身长、体重、及钙存留率显著相关（Ｐ＜０．０５）；ＢＭＣ与钙总摄入量、身长、体重、性别和肝脏钙含量显著相关（Ｐ＜０．０５）。多元回归分析表明：动物身长、体重、钙总摄入量和心脏钙含量与活体ＢＭＤ呈线性关系，动物身长和血浆钙含量与离体ＢＭＤ呈线性关系。活体测量结果ＢＭＣ、ＢＭＤ由于其与动物的生长发育、钙存留率及肌肉和血浆钙含量具有相关和直线关系，且简便易行、不需要处死动物，因而认为可以反映动物体内钙营养状况和骨质积累情况。

A Study on the Relationship between Bone Mineral Content and the Tissue Calcium Level in Rats

The relationship of bone mineral density (BMD) and bone mineral content (BMC) of femur bone with the tissue and blood calcium contents was studied with 98 Wistar rats (15 week old). The results indicated that the body length, body weight, bone width, BMC, BMC/weight, BMD/weight and BMD/length in male were markedly different from those in female animals. The BMD of alive rats was significantly correlated with the total calcium intake, length, weight and calcium retention rate (P<0.05). BMC was significantly related to sex, length, weight, liver calcium content and total calcium intake. Multiple regression analysis indicated that the animal body length, weight, total calcium intake and calcium content in heart were positively correlated with the BMD of alive animals. The animal body length and plasma calcium content were positively correlated with BMD of separated bones after the animals were sacrificed. The present results indicated that the BMC and BMD of alive rats could reflect the calcium status and bone mineral accumulation in the body because of the significant correlation with the growth rate, calcium retention rate, muscle and plasma calcium contents, but not with the separated bone BMD or BMC.