腺苷对人静脉内皮细胞增殖的影响

目的探讨腺苷对人脐静脉内皮细胞（human umbilical vein endothelial cell，HUVEC）增殖的影响。方法MTT法观察腺苷的最佳作用浓度及最佳作用时问。结果4％FBS浓度下，腺苷具有明显的促HUVEC增殖作用，为最佳浓度。在10-^4 mol／L腺苷终浓度对细胞增殖有明显的促进作用，为最佳腺苷浓度。腺苷的促生艮作用在24h即出现，在48h已达到较高水平。结论在血供基本被阻断或血流基本正常的情况下，腺苷可能对细胞增殖尤影响；但在m流减少的情况下，腺苷的应用可以促进内皮细胞增殖，可能对血管新生有启动作用。

Influence of adenosine on proliferation of human umbilical vein endothelial cell

Objective To explore the influence of adenosine on proliferation of human umbilical vein endothelial cell (HUVEC).Methods MTT technique was used to observe the optimal concentration and action time.Results 4% fetal bovine serum (FBS) was the optimal concentration for adenosine with significant role of promoting HUVEC proliferation,while 10 -4 mol/L adenosine was the optimal concentration for its significant role of promoting HUVEC proliferation. The effect of adenosine on promotion of HUVEC proliferation appeared at the 24th hour and reached the higher level at the 48th hour.Conclusions When the blood supply is nearly completely blocked or nearly normal,adenosine has no influence on HUVEC proliferation,but when blood supply is diminished,adenosine may promote proliferation of HUVEC and provide opportunity for the onset of angiogenesis.