高效液相色谱法测定左旋多巴／苄丝肼分散片血药浓度及其药代动力学研究

目的建立左旋多巴血药浓度的测定方法并对其分散片的人体药代动力学进行研究。方法采用反相高效液相色谱法，色谱柱为Lichrospher100C

HPLC determination oflevodopa in human plasma for study of the pharmacokinetics of levodopa/benserazidedispersible tablets

Objective:During study of pharmacokinetics of levodo pa/benserazide dispersible tablets，a HPLC method for determination of levodopa in human plasma was developed.Methods:Waters 2010 system was adopt ed with a 474 fluorecence detector(λEx=278nm,λEm=325nm),a Lichrosp her 100 C18 column(5μm,250 mm×4 mm),a mobile phase (pH 3.7) consisted of 90% solution (containing EDTA 0.08 mmol/L,KH2PO4 70 mmol/L and sodium hepta nesulfonate 2.08 mmol/L)and 10% methanol and using a flow rate of 1.0 ml/min.Aft er deproteinized with perchloric acid,plasma sample was directly injected. Results:The method have a recovery range of 95.50%～102.55%,a minimum d etection limit of 0.25ng,a linear range of 0.125～4.0 μg/ml within-day RSD<4.2 9%(n=4),between-day RSD<6.73%(n=4).After oral administration of 200 mg of levodopa/benserazide dispersible tablet,the disposition was conformed to a two-compartment model with AUC=(406.09±85.02)(μg*min)/ml,C max=(3.55±0.91)μg/ml,Tmax=(31.38±7.74) min and t1/2α=(24.09±6 .02)min.Compared with levodopa tablet,the Tmax and t1/2α showed sta tistical difference.The relative bioavailability was (97.63±11.94)%.Con clusion:A s imple,sensitive and accurate method for HPLC determination of levodopa in human plasma was developed,and levodopa/benserazide dispersible tablet was absorbed an d reached peak-time quickly than levodopa tablet.the method has been used in th e study of pharmacokinetics of levodopa/benserazide dispersible tablets.