两种染色方法对神经元显示效果的比较

①目的探讨理想的显示神经元微细结构的方法。②方法取初生小牛脊髓横断切片，分别采用硫瑾-伊红与苏木精-伊红染色方法显示神经元结构。③结果硫瑾-伊红染色后神经元胞核大而圆，染色浅，核仁明显，胞体及树突内可见紫蓝色尼氏体分布。苏木精-伊红染色．胞核不清楚，尼氏体不明显，轴突与树突难以区分。④结论光镜下硫瑾-伊红染色比苏木精伊红染色更能清楚显示神经元的结构特征。

A COMPARISON BETWEEN THIONIN-EOSI N STAINING AND HEMATOXYLIN-EOSIN TECHNIQUE FOR SHOWING NEURONS

Objective\ To explore an ideal method for showing the histological structure of the neurons.\ Methods Cross sections of spinal cord removed from neonate cows were stained with thionin eosin (H E) or hematoxylin eosin respectively to show the cellular structure of the neurons.\ Results\ With thionin eosin staining, the neuron demonstrated a single, large and pale nucleus with a distinct nucleolus, and the Nissl bodies appeared as distinct basophilic masses which distributed symmetrically within the perikaryon and dendrites. When stained with H E, the nucleus was not clear, Nissl bodies were not distinct and the axon and dendrites could not be distinguished. \ Conclusion\ Thionin eosin staining technique is more sensitive than H E method in showing characters of neurons under light microscope.