大鼠端脑与间脑内糖皮质激素受体免疫反应性神经原的分布

应用抗大鼠肝脏糖皮质激素受体(GR)的单克隆抗体结合间接免疫酶标(PAP)技术对大鼠端脑与间脑GR免疫反应性神经细胞与胶质细胞的分布进行了研究。强GR免疫反应性染色可见于下丘脑室旁核小细胞部(fp),下丘脑内侧基底部弓状核和海马结构的CA1,CA2亚区。整个大脑皮层(尤其是第Ⅳ层)有中等到高密度的GR免疫反应性神经细胞核分布,呈轻度染色。许多丘脑核与中央杏仁核有中等密度的GR免疫反应性神经细胞核分布,轻度着色。经双侧去肾上腺大鼠,GR免疫反应性仅出现在核周胞质内;若急性投予皮质酮,则在胞核内出现相当浓的GR免疫反应性染色。此实验结果提示皮质酮可作为特异性配体与GR结合,然后皮质酮—GR复合物转移入细胞核;符合传统的类固醇激素受体的作用机理。

Mapping of Glucocortioid Receptor Immunoreactive Neurons in the Rat Tel-and Diencephalon

By means of a monoclonal antibody against the rat liver glucocorticoid receptor (GR) in combination with the indirect immunoperoxidase technique, it has been possible to demonstrate CR immunoreactive nerve and glial cell nuclei all over the tel-and diencephalon of the male rat. Strongly CR immunoreactive nerve cell nuclei only present in the parvocellular part of the paraventricular hypothalamic nucleus (fp) , in the ventral part of the mediobasal hypothalamus and in the CA1 and CA2 subregions of the hippocampal formation. Medium to high densities of moderately GR immunoreactive nerve cell nuclei were present all over the cortical hemispheres.Medium densities of moderately GR immunoreac-tive nerve cell were demonstrated in many thalamic nuclei and in the central amygdaloid nucleus.Following adrenalectomy, the GR immunoreactivity was predominantly located in the pericaryon. Upon acute corticosterone treatment of ad-renalectomized male rats,the GR immunoreactivity was again mainly demonstrated in the nuclei indicating that corticosterone can translocate GR from the cytoplasma to the cell nuclei.