AFLP技术及其应用

介绍扩增的片段长度多态性技术及其在分子流行病学中的应用，对近年来ＡＦＬＰ技术在几种病原菌的应用进行综述，并与其它分子流行病学方法进行比较。ＡＦＬＰ具有其它基因指纹图谱方法的优点，并且相对快速、简便，结果可重复，分辨力高。ＡＦＬＰ是一种基于ＰＣＲ的高分辨ＤＮＡ指纹方法，必将广泛应用于微生物的分型与鉴定。

Amplified Fragment Length Polymorphism (AFLP) and its Applications

The principle of amplified fragment length polymorphism (AFLP) and its application in molecular epidemiology are introduced. The application of AFLP in several pathogens (such as Legionella pneumophila, Flavobacterium, Aeromonas, Clostridium, Bacillus, Acinetobacter, Pseudomonas, Vibrio, Helicobacter pylori and Group A Streptococcal) is reviewed and especially compared with other molecular epidemiological methods. AFLP shares many characteristics with other genetic fingerprinting methods. In addition, it is relatively rapid, technically simple and gives reproducible and discriminatory results. AFLP is a high resolution PCR based fingerprinting method that will be widely used in typing and identifying of microorganisms.