口腔内水刺激的脑功能成像研究

目的利用功能性磁共振成像（fMRI）技术探讨高级中枢如何解读口腔内水刺激。方法选取13名健康志愿者，利用自制水传递装置将水注入口腔内，采用水刺激信号减静息信号的组块设计方法，以SPM99软件分析数据获得口腔内水刺激与静息状态对比的fMRI脑功能图像。结果水刺激口腔时，广泛激活左侧岛叶、罗兰多区（BA1/3/4/43）、右侧眶额皮层（BA11）、额中回（BA10），其次是右侧顶叶（BA7）、中脑红核、扣带回和左侧额下回、岛叶（BA13）。结论口腔内水刺激引起口腔感觉运动代表区的激活，还引起其他相关脑区激活；在研究味觉和温度觉的神经解剖研究过程中需要控制水和其他非味觉因素。

Cortical activation induced by intraoral stimulation with water in humans with functional magnetic resonance imaging

OBJECTIVE: To investigate the neural representations of normal humans intraoral water stimulation using non-invasive functional magnetic resonance imaging (fMRI) measurement, provide information for intraoral thermosensory study. METHODS: 13 healthy right-handed subjects (of which 6 were males) participated in the study to receive 23 degrees C deionized distilled water stimuli in mouth. The experimental protocol consisted of a block-design using 5 stimulus. Water stimuli were delivered to the subject's mouth through self-made polythene tubes that were held between lips. Functional MRI scan covering the whole brain was carried out. The fMRI data was analyzed by SPM99 software with statistic t-test to generate the activation map. RESULTS: Significant activations existed in peri-sylvian including pre/ post-central gyrus, insular, orbitalfrontal cortex (OFC), anterior cingulate cortex (ACC), frontal gyrus, parietal lobule, midbrain and parietal lobule. CONCLUSION: Water's ability to activate nonspecific somatosensory, thermal or taste processing. The findings highlight the complex and distributed processing of different aspects of intraoral perception, and emphasize the importance of controlling for the effects of water when investigating the functional neuroanatomy of taste or temperature.