RP-HPLC法测定骨疏丹颗粒中5种有效成分的含量

目的采用RP-HPLC法同时测定骨疏丹颗粒剂中新北美圣草苷、柚皮苷、淫羊藿苷、欧前胡素、蛇床子素5种活性成分。方法采用Diamonsil C18色谱柱(250 mm×4.6 mm,5μm),以乙腈-水为流动相,梯度洗脱,流速1.0 mL.min-1,柱温为室温,检测波长285 nm。结果各个成分的峰面积与其质量浓度的线性关系良好(r>0.9990),加样回收率为95.8%~98.6%。结论该方法简单、准确、重现性好,可为全面评价复方骨疏丹颗粒剂的质量提供依据。

Determination of five active constituents in Gushudan granules by RP-HPLC

Objective To determine the contents of the neoeriocitrin, icariin, osthole, naringin, imperatorin in Gushudan granules ( traditional Chinese medicine ). Methods Diamondsil TM C₁₈ (250 mm × 4.6 mm, 5 μm) column was used with a mobile phase of acetonitrile-water; the flow rate was 1.0 mL·min^{- 1} ; the detection wavelength was set at 285 nm. Results The linearity was obtained with good result (r<0.999 0). The average recoveries of five active constitutes in Gushudan granules were all in 95.8%- 98.6%, and the RSD were less than 2.1%. Conclusion The validated method has the advantages of simplicity, precision and reliability, allowing quality control of Gushudan granules.