| 神经干细胞分化的神经元与尾状核神经元共培养的实验研究 |
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| 引用本文： | 唐洲平,王萍,许峰,康慧聪,陈玲,朱遂强,张苏明,方思羽. 神经干细胞分化的神经元与尾状核神经元共培养的实验研究[J]. 卒中与神经疾病, 2005, 12(4): 195-197 |
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| 作者姓名： | 唐洲平 王萍 许峰 康慧聪 陈玲 朱遂强 张苏明 方思羽 |
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| 作者单位： | 1. 430030,武汉,华中科技大学同济医学院附属同济医院神经内科
2. 湖北省恩施自治州中心医院麻醉科 |
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| 摘 要： | 目的研究大鼠神经干细胞(NSCs)分化后与尾状核神经元共培养时是否形成突触连接.方法分离培养大鼠NSCs及尾状核神经元,用绿色荧光蛋白重组腺相关病毒(eGFP)转染NSCs;将eGFP-NSCs 分化后和大鼠尾状核神经元共培养,利用免疫细胞化学方法和激光共聚焦显微镜分析结果.结果 eGFP-NSCs分化的神经元与尾状核神经元共培养时,两者有突触连接形成.结论 NSCs分化的神经元具有形成神经网络的能力.
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| 关 键 词： | 神经干细胞 尾状核神经元 eGFP转染 突触 大鼠 |
| 文章编号： | 1007-0478（2005）04-0195-03 |
| 收稿时间： | 2005-04-29 |
| 修稿时间： | 2005-04-29 |
The experiment study of co-culture of the neurons differentiated from neural stem cells and neurons from originated rat caudate necleus |
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| Tang ZhouPing;Wang Ping;Xu Feng;Kang HuiCong;Chen Ling;Zhu SuiJiang;Zhang SuMing;Fang SaiYu. The experiment study of co-culture of the neurons differentiated from neural stem cells and neurons from originated rat caudate necleus[J]. Stroke and Nervous Diseases, 2005, 12(4): 195-197 |
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| Authors: | Tang ZhouPing Wang Ping Xu Feng Kang HuiCong Chen Ling Zhu SuiJiang Zhang SuMing Fang SaiYu |
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| Abstract: | Objective To study whether there were synapses formatted when the neurons differentiated from the rats NSCs and neurons originated from the caudate nucleus were mixed cultured. Methods Separating and culturing rats NSCs and neurons originated from rat caudate nucleus, we have transected eGFP into NSCs, co-cultured the neurons derived from the NSCs and neruons originated from the caudate nucleus, then analyzed the results with immunocytochemistry test and Confocal Laser Scanning Microscopy. Results The progeny of eGFP-NSCs could develop into neurons with synapse, when co-cultured with the primary neurons. Conclusions Neurons differentiated from eGFP-NSCs can make synapses with neurons originated from caudate nucleus when they are mixed cultured. |
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| Keywords: | Neural stem cells Neurons of Caudate Nucleus eGFP Transfection Synapse rats |
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