氯沙坦对家兔早期动脉粥样硬化血管MCP-1表达及胆固醇含量的影响

目的 :氯沙坦对加速性家兔早期动脉粥样硬化 （AS）血管增生及 MCP- 1蛋白表达的影响。方法 :采用组织学、免疫组织化学和生物化学方法评价加速性家兔 AS早期血管组织学、增殖细胞核抗原 （PCNA）及单核白细胞趋化蛋白 - 1（MCP- 1）蛋白表达和组织胆固醇含量的改变。结果 :AS组血管内膜与中膜厚度比值、主动脉组织胆固醇含量、内膜 PCNA阳性细胞数及 MCP- 1蛋白表达平均光密度积分值均显著增加 ,氯沙坦干预组内膜与中膜厚度比值较 AS组显著下降 ,PCNA阳性细胞数明显减少 ,血管壁 MCP- 1蛋白表达水平明显下降 （均 P<0 .0 1）。氯沙坦干预组主动脉组织胆固醇含量明显低于 AS组 1.47± 0 .0 3mg/ g（wet）与 1.35± 0 .0 2 mg/ g（wet） ,P<0 .0 5 ]。结论 :氯沙坦干预可抑制 MCP- 1蛋白表达 ,减轻加速性 AS病变血管内膜增生 ,减少胆固醇在血管壁沉积 ,从而可能在防治AS过程中起重要作用。

Effects of losartan on expression of monocyte chemoattractant protein-1 and aortic cholesterol content in a rabbit model of early accelerated atherosclerosis

AIM:To investigate effects of losartan on aortic cholesterol content and expression of monocyte chemoattractant protein-1(MCP-1) in a rabbit model of early accelerated atherosclerosis. METHODS:Aortic cholesterol content,expression of proliferation cell nuclear antigen(PCNA) and MCP-1 protein were observed by enzymatic analysis and immunohistochemistry. RESULTS:The ratio of vascular intimal to medial thickness,aortic cholesterol content,numbers of positive PCNA cells,and mean A values of expression of MCP-1 protein in AS group were singnificantly increased than in control group,losartan-pretreated rabbits displayed a significant decrease of ratio of vascular intimal to medial thickness,numbers of positive PCNA cells and mean A values of expression of MCP-1 protein(P<0.01). Aortic cholesterol content in losartan-pretreated group were significantly decreased than that in AS group (P<0.05). CONCLUSION:Losartan inhibits vascular MCP-1 expression,decreases cholesterol deposit in vascular walls and inhibits intimal proliferation,which may play an important role in prevention of atherosclerosis.