Evaluation of the anti-candidal activity of methanolic leaf extract of cleistopholis patens (fam. Annonaceae) on candida species isolated from stage II HIV patients |
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Authors: | David C Okechukwu Mumuni A Momoh Charles O Esimone |
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Institution: | 1 Pharmaceutical Microbiology Unit of Department of Pharmaceutics, Faculty of Pharmaceutical Sciences University of Nigeria, Nsukka, Enugu State, Nigeria;2 Department of Pharmaceutical Microbiology and Biotechnology Nnamdi Azikiwe University, Awka, Anambra State, Nigeria |
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Abstract: | BackgroundCandida species (sp) is implicated in causing opportunistic disseminated mycotic complications in stage II HIV patients. Cleistopholis patens is a West African medicinal tree reported to have significant antifungal activity against C. albicans.ObjectivesThis study aimed to determine the anti-candidal activity of methanolic leaf extract of Cleistopholis patens against Candida species isolated from stage II HIV patients.MethodsThe minimum inhibitory concentration (MIC) of the extract and Nystatin®® was determined by agar dilution method. The killing rate studies of the plant extract and Nystatin® were also determined.ResultsThe extract had activity against all Candida isolates, with the MIC against the five isolates ranging from 6.0 – 9.8 mg/ml. Nystatin® also demonstrated plausible activity against the isolates with MICs ranging from 0.3125 — 25 mg/ml. Candida albicans strain 2 was the most sensitive to both extract and Nystatin® with MIC values of 6 and 0.3125 mg/ml respectively. Candida krusei was the least sensitive with MIC values of 9.8 and 25 mg/ml for the extract and Nystatin® respectively. The killing rate values for the extract ranged from −0.029 to −0.091 min−1 and that of Nystatin® ranged from −0.076 to −0.11216 min−1.ConclusionsThe results indicate that the methanolic extract of Cleistopholis patens is a promising clinical alternative besides Nystatin® in the treatment of infections caused by Candida species in stage II HIV patients. |
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Keywords: | Anti-candidal activity Cleistopholis patens Candida HIV Patients |
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