Tryptophan-induced pathogenesis of breast cancer |
| |
| Authors: | Zhu-Gen Cao Xiao-Bing Qin Fang-Fang Liu Lei-Lei Zhou |
| |
| Affiliation: | 1 Department of Oncology, Si''hong County People''s Hospital, Si''hong, Jiangsu, 223900, P.R. China;2 Department of Oncology, The First People''s Hospital of Xuzhou, Xu''zhou, Jiangsu, 221002, P.R. China;3 Department of General Surgery, Huai''an Second People''s Hospital, Huai''an, Jiangsu223002, P.R. China;4 Department of Oncology, Huai''an First People''s Hospital, Nanjing Medical University, Huai''an, Jiangsu 223300, P.R.China |
| |
| Abstract: | BackgroundThe pathogenesis of breast cancer remains unclear.AimsTo investigate the pathogenesis of breast cancer through targeted metabolomics of amino acids components in serum of patients with breast cancer.MethodsPatients with breast cancers were enrolled in our hospital between year January 1st, 2013 and December 31st, 2014. Targeted analysis of amino acids was performed using ESI-QTOF-MS instrument. In vitro experiment was performed to determine the influence of tryptophan towards interleukin-10 (IL-10) secretion by CD4+ T cell.ResultsTargeted metabolomics of amino acids showed that the level of tryptophan significantly (p<0.05) increased in patients with breast cancer. Furthermore, the biological function of tryptophan was determined through determining the influence of tryptophan towards IL-10 secretion using in vitro method. The addition of tryptophan (100 uM) in the cell medium can significantly inhibited the secretion of IL-10 by CD4+ T cells, as indicated by the mRNA level and protein concentration.ConclusionThe inhibition of IL-10 secretion by CD4+ T cells is a potential pathogenesis of breast cancer. |
| |
| Keywords: | breast cancer interleukin-10 (IL-10) pathogenesis T cell |
|
|
