牙本质涎蛋白在氟中毒大鼠牙髓组织和牙本质中的表达和意义

目的:研究氟中毒对大鼠牙髓及牙本质表达DSP的影响。方法:选择20只Wistar大鼠,随机分为对照组(饮用自来水,水氟浓度0.16 mg/L)和给氟组(水氟浓度100 mg/L)。3个月后处死大鼠,利用HE染色、免疫组化染色观察氟中毒对牙本质结构和DSP表达的影响。结果:100 mg/L组牙本质生长线明显加重,出现大量球间牙本质。DSP蛋白在牙本质、成牙本质细胞、牙髓细胞中均有不同程度的表达,在牙本质层内侧的成牙本质细胞和牙髓细胞的染色强度2组间无明显区别(P>0.05)。在给氟组,强烈的DSP染色持续出现在前期牙本质层和矿化的牙本质层(P<0.05),表现为加重的生长线。结论:DSP在氟中毒组牙本质中表达明显增强,推测氟可能影响DSP蛋白的降解,影响牙本质的正常矿化。

The expression and significance of dentin sialoprotein in rat pulp and dentine during fluorosis

Objective: To study the expression and significance of dentin sialoprotein(DSP) during fluorosis in rat pulp and dentine.Methods: Rats were exposed to 0.16 mg/L of water fluoride ion concentration in control group and 100 mg/L in fluoride group for 3 months,then the animals were in killed.HE staining was used to observe the morphology of the teeth,immunohistochemical staining was adopted for the observation of DSP expression.Results: In the 100 mg/L NaF treated group,the large interglobular spaces were observed between unmerged calcospherites.Marked enhancement of the incremental patterns were seen in dentine.DSP expression in pre dentine and mineralized dentine was increased(P<0.05),but in odontoblast,pulp tissue showed no significant changs after administration of fluoride(P>0.05).Conclusion: Fluoride may increase DSP expression in dentin and interfere the mineralisation process.