钙调神经磷酸酶在哮喘豚鼠气道重塑中的作用

目的:观察钙调神经磷酸酶(CaN)在哮喘豚鼠气道重塑中的作用。方法:实验分3组:对照组、哮喘组及CaN抑制剂环孢霉素(CsA)组,测定指标包括:①支气管肺泡灌洗液(BALF)蛋白含量、细胞计数及分类;②大气道平滑肌³H]-TdR掺入量;③肺组织切片中小气道壁厚度及气道平滑肌厚度;④气管和肺组织CaN活性。结果:①BALF:CsA组蛋白含量、细胞计数及嗜酸粒细胞分别较哮喘组少46%、51%及60%(P＜0.01);②大气道平滑肌³H]-TdR掺入量:CsA组较哮喘组低22%(P＜0.05);③小气道壁厚度:CsA组较哮喘组少34%(P＜0.01);气道平滑肌厚度:CsA组较哮喘组少37%(P＜0.01);④肺组织CaN活性:CsA组较哮喘组低52%(P＜0.01);气管CaN活性:CsA组较哮喘组低44%(P＜0.01)。结论:CsA可减轻哮喘豚鼠气道重塑,推测CaN参与气道重塑过程。

Role of calcineurin in airway remodeling in guinea pig model of asthma

AIM: To investigate the role of calcineurin (CaN) in airway remodeling in guinea pig model of asthma.METHODS: Male guinea pigs were randomly divided into three groups: control, asthma group and CsA group. The following parameters were measured: 1. The protein content, cell count and differential count of BALF; 2. The amount of TdR incorporation into central airway smooth muscle; 3. The mean thickness of airway wall and airway smooth muscle of small airwaysl; 4.CaN activity of trachea and lung tissue.RESULTS: 1. The protein content, cell count and eosinophil of BALF in CsA group were 46%, 51% and 60% lower than those in asthma group, respectively ( P <0 01); 2. TdR incorporation in CsA group was 22% lower than that in asthma group ( P <0 05);3. The mean thickness of airway wall and airway smooth muscle were 34% and 37% less in CsA group than those in asthma group, respectively ( P <0 01); 4. CaN activity of lung tissue and trachea were 52% and 44% lower in CsA group than those in asthma group, respectively ( P <0 01).CONCLUSION: CsA reduced airway remodeling in guinea pig model of asthma, indicating the role of CaN in the airway remodeling.