| 磷酸二酯酶4抑制剂Hemay005对角质形成细胞炎性因子产生的影响 |
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| 引用本文： | 宋莎莎,李新宇,王永芳,朱菁,黄俊哲,张和胜,艾涛,裴书捷.磷酸二酯酶4抑制剂Hemay005对角质形成细胞炎性因子产生的影响[J].中国中西医结合皮肤性病学杂志,2014(1):9-12. |
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| 作者姓名： | 宋莎莎 李新宇 王永芳 朱菁 黄俊哲 张和胜 艾涛 裴书捷 |
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| 作者单位： | [1] 中国医学科学院/北京协和医学院皮肤病研究所,南京210042 [2] 天津和美生物技术有限公司,天津300192 [3] 海南海灵化学制药有限公司,海口570311 |
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| 摘 要： | 目的 为了探讨磷酸二酯酶4抑制剂Hemay005对体外培养的角质形成细胞株HaCaT细胞炎性因子产生的影响.方法 分别用重组人肿瘤坏死因子-α（rhTNF-α）、重组人干扰素-γ（rhIFN-γ）和312 nm窄波UVB诱导,采用MTT法检测Hemay005对HaCaT细胞增殖的影响,用ELISA法检测其对HaCaT细胞白细胞介素（IL）-8、sICAM-1和肿瘤坏死因子（TNF）-α产生的影响.结果 Hemay005对HaCaT细胞增殖及25 μg/L rhTNF-α诱导的IL-8产生无明显影响,但能剂量依赖性地抑制1 000 U/mL rhIFN-γ诱导的HaCaT细胞产生sICAM-1和124.2 mJ/cm2窄波UVB照射引起的HaCaT细胞产生TNF-α.结论 Hemay005可通过抑制角质形成细胞炎症因子的表达发挥抗炎作用.
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| 关 键 词： | 磷酸二酯酶4抑制剂 白细胞介素-8 可溶性细胞间黏附分子-1 肿瘤坏死因子-α Interleukin-8 Soluble intercellular adhesion molecule-1 Tumor necrosis factor-α |
Effects of Phosphodiesterase 4 Inhibitor Hemay005 on the Expression of Inflammatory Factors in Human Keratinocytes |
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| SONG Shasha,LI Xinyu,WANG Yongfan,ZHU Jing,HUANG Junzhe,ZHANG Heshen,AI Tao,PEI Shujie.Effects of Phosphodiesterase 4 Inhibitor Hemay005 on the Expression of Inflammatory Factors in Human Keratinocytes[J].Chinese J of Dermatovenerology Integr Tradit and West Med,2014(1):9-12. |
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| Authors: | SONG Shasha LI Xinyu WANG Yongfan ZHU Jing HUANG Junzhe ZHANG Heshen AI Tao PEI Shujie |
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| Institution: | 1.1nstitute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing 210042, China; 2.Tianjin Hemay Bioltechnology Corporation Limited, Tianjin 300192, China; 3.Hainan Hailing Chemipharma Corporation Limited, Haikou 570311, China) |
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| Abstract: | Objective To investigate effects of phosphodiesterase 4 inhibitor Hemay005 on the proliferation of keratinocytes and expression of inflammatory factors. Methods HaCaT keratinoeytes were induced by recombinant human TNF-α （rhTNF-α, recombinant human IFN-γ （rhIFN-γ） and 312 nm UVB, respectively. The proliferation of HaCaT cells were detected by MTF method, the production of IL-8, sICAM-1 and TNF-α in HaCaT cells were determined by ELISA. Results It showed that HemayO05 had no significant influence on the proliferation of HaCaT cells and the production of IL-8 induced by 25 ng/mL rhTNF-α, but it could inhibit the production of sICAM-1 induced by 1000 U/mL rhIFN-γand TNF-α stimulated by 124.2 mJ/cm^2 narrowband UVB in a dose-dependent manner. Conclusion Hemay005 might play the role of antipsoriasis by inhibiting the expression of inflammatory factors. |
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| Keywords: | Phosphodiesterase 4 inhibitor Interleukin-8 Soluble intercellular adhesion molecule-1 Tumor necrosisfactor-α |
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