肝/肝干细胞移植研究中的若干问题探讨

肝细胞移植以其移植技术简单、可重复进行、对受体影响小以及一肝多用、免疫排斥反应弱等优点为肝脏衰竭的治疗提供了一种全新的治疗策略，尤其是近年来肝干细胞的研究进展为这一技术由实验室过渡到临床提供了新的研究思路。本文以笔者实验室自1998年至今的连续的研究工作为基础，回顾了其发展历史，并围绕移植细胞的来源、细胞移植技术、细胞移植机制、移植细胞的示踪等四个方面问题，结合文献进行讨论。移植细胞的来源主要讨论了肝细胞和肝干细胞；细胞移植技术主要为脾内移植技术；在细胞移植机制方面，主要探讨受体肝卵圆细胞的作用；移植细胞的示踪方面主要是应用磁探针标记和磁共振技术进行肝干细胞的示踪。

Several key issues on hepatocyte/hepatic stem cell transplantation

Hepatocyte transplantation could be a new pathwa y fo r the treatment of liver diseases, with its advantages of simple technique, repe ated procedures, instant usage on-demand, and little immunoreactions. The recen t progress on study of hepatic stem cells shows a promising future for this tech nique. Based on the investigations in authors' laboratory, this article reviews the history and current status by focusing on resources of hepatocytes/hepatic s tem cells, intrasplenic hepatocyte transplantation, action of hepatic oval cells in liver cell transplantation, and MRI tracing technique by labeling transplant ed stem cells with magnetic particles.