电针对压力性尿失禁大鼠盆底组织胶原蛋白表达的影响

目的通过观察压力性尿失禁（SUI）大鼠盆底支持组织Ⅰ和Ⅲ型胶原蛋白含量以及Ⅰ/Ⅲ比值的变化,探讨电针对压力性尿失禁大鼠盆底支持组织胶原蛋白影响,初步阐释针灸治疗压力性尿失禁的部分机制。方法将大鼠施阴道扩张和双侧卵巢切除为SUI动物模型,应用酶联免疫法,观察尿道下阴道壁、耻骨尿道韧带、耻骨尾骨肌和尿道周围结缔组织Ⅰ和Ⅲ型胶原蛋白含量以及Ⅰ/Ⅲ比值的变化。结果与结论电针可增加压力性尿失禁大鼠盆底支持组织Ⅰ和Ⅲ型胶原蛋白含量以及Ⅰ/Ⅲ比值;电针对压力性尿失禁具有良好调整作用,影响压力性尿失禁大鼠盆底支持组织Ⅰ和Ⅲ型胶原蛋白含量以及Ⅰ/Ⅲ比值。最终实现对压力性尿失禁的调整。

Effect of Electroacupuncture on the Expressions of Collagens in the Pelvic Floor Tissue of Stress Urinary Incontinence Rats

Objective To investigate the effect of electroacupuncture on collagen in the pelvic floor supporting tissue by examining changes in type 1 and 3 collagens and the ratio of type 1 to 3 in the pelvic floor supporting tissue of stress urinary incontinence （SUI） rats and to preliminarily explain part of the mechanism of acupuncture treatment for stress urinary incontinence. Methods A rat model of SUI was made by colpeurysis and bilateral ovariectomy. Changes in type 1 and 3 collagen contents and the ratio of type 1 to 3 in the suburethral vaginal wall,pubourethral ligament,pubococcygeus and periurethral connective tissue were examined by enzyme linked immunosorbent assay. Results and conclusion Electroacupuncture increased type 1 and 3 collagen contents and the ratio of type 1 to 3 in the pelvic floor supporting tissue of stress incontinence rats. Electroacupuncture has a good therapeutic effect on stress incontinence. It influences type 1 and 3 collagen contents and the ratio of type 1 to 3 in the pelvic floor supporting tissue of stress incontinence rats so as to treat stress urinary incontinence.