反义hTERT对胃癌细胞SGC7901 VEGF及其受体表达的影响

目的研究反义hTERT对胃癌细胞SGC7901 VEGF及其受体表达的影响,探讨其对肿瘤发展和转移的影响.方法以端粒酶逆转录酶组分hTERT cDNA为模板构建反义hTERT逆转录病毒表达载体,包装重组逆转录病毒后感染胃癌SGC7901细胞,用半定量RT-PCR检测基因转染前后VEGF-C及其受体Flt-4 mRNA表达水平的变化,免疫组化法检测VEGF-C、Flt-4蛋白表达.结果反义hTERT稳定转染后SGC7901细胞VEGF-C及其受体VEGFR-3（Flt-4） mRNA和蛋白表达均受到明显抑制.结论反义hTERT可有效抑制胃癌细胞VEGF-C及其受体Flt-4的表达,阻断肿瘤血管和淋巴途径转移.

The influence of anti-sense hTERT on the expression of VEGF and Flt in SGC7901 cells

Objective To investigate the effects of anti sense hTERT on the expression of VEGF and the receptor in SGC7901 cells. and study the influence in angiogenesis and progression in gastric carcinoma. Methods SGC7901 cell line was transfected by the recombinant virus containing sense and anti-sense hTERT cDNA. Then the expression of VEGF-C and Fit 4 was observed with RT-PCR, distribution of cell cycles was determined with flow cytometry. The expression of VEGFC and Fit 4 protein was assessed with immunohisto- chemistry. Result The distribution of cell cycle of antisense hTERT transfected SGC7901 cells was changed, and the mRNA and protein expression of VEGFC and Flt 4 was significantly down regulated. Conclusion Anti sense hTERT can act as an agent for inhibiting VEGF-C and Flt-4 mRNA and protein level, and it blocks tumor angiogenesis and lympbogenous metastasis.