| 携带野生型p53基因的重组腺病毒载体的构建 |
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| 引用本文： | 尹新华,凌虹.携带野生型p53基因的重组腺病毒载体的构建[J].哈尔滨医科大学学报,1997,31(6):441-443. |
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| 作者姓名： | 尹新华 凌虹 |
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| 摘 要： | 应用同源重组方法成功地构建了携带野生型Ｐ５３基因的复制缺陷型重组腺病毒，通过光镜电镜、酶切、ＰＣＲ共扩增等方法证实了构建载体的正确性，并使常规的同源重组方法进一步改进，简化了载体构建的操作程序，结果准确，成功率高。本结果可用于以腺病毒为载体进行肿瘤、心血管疾病等基因治疗的研究。
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| 关 键 词： | 腺病毒 载体 同源重组 p53基因 基因疗法 |
Construction of Recombinant Adenoviruses Vector that Coding Wild Type p53 Gene |
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| Yin Xinhua et al.Construction of Recombinant Adenoviruses Vector that Coding Wild Type p53 Gene[J].Journal of Harbin Medical University,1997,31(6):441-443. |
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| Authors: | Yin Xinhua |
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| Institution: | Instiute of Keshan Disease |
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| Abstract: | A replication defective recombinant adenoviruses that coding wild type p53 gene were constructed by means of homologous recombination.The structure of the recombinant adenovirus were confirmed by light and electronic microscopy,restriction analysis and PCR co amplication.Our procedure simplified the routine protocol of homologous recombinant with improved result.This work could be used in the therapy of tumor and cardiovascular diseases which mediated by recombinant adenoviruses. |
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| Keywords: | Gene therapy Adenovirus vectors Homologous recombinant p53 gene |
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