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微柱凝胶卡式法在交叉配血及不规则抗体检测中的应用
引用本文:叶巧国,杨毓,何静,张卫良,李交,梁丽雯.微柱凝胶卡式法在交叉配血及不规则抗体检测中的应用[J].现代医院,2011,11(12):59-61.
作者姓名:叶巧国  杨毓  何静  张卫良  李交  梁丽雯
作者单位:1. 佛山市顺德区陈村医院,广东佛山,528300
2. 佛山市顺德区中心血站,广东佛山,528300
摘    要:目的研究微柱凝胶卡式法在临床交叉配血及不规则抗体检测中的应用。方法采用微柱凝胶卡式法进行交叉配血,对配血结果呈特异性凝集的阳性标本进一步作不规则抗体检测,再选取不规则抗体相应抗原阴性的献血者与患者进行交叉配血。结果在1 051例配血标本中,交叉配血阳性结果有27例,在不规则抗体检测中检出抗-E 3例,抗-c 1例,抗-cE 1例,抗-M 1例,选取不规则抗体相应抗原阴性的献血者与患者进行交叉配血,配血成功。其余21例为非特异性抗体或自身抗体。结论微柱凝胶卡式法是交叉配血试验、不规则抗体筛查的最新方法,应该在临床大力推广使用。

关 键 词:微柱凝胶卡式法  交叉配血  不规则抗体  输血  筛检标准红细胞  谱细胞

APPLICATION OF MICROCOLUMN GEL TECHNIQUE IN CROSS MATCHING AND IRREGULAR ANTIBODIES DETECTION
YE Qiaoguo,YANG Yu,HE Jing,et al Chencun Hospital of Shunde District,Foshan City,Guangdong Province PRC.APPLICATION OF MICROCOLUMN GEL TECHNIQUE IN CROSS MATCHING AND IRREGULAR ANTIBODIES DETECTION[J].Modern Hospital,2011,11(12):59-61.
Authors:YE Qiaoguo  YANG Yu  HE Jing  Chencun Hospital of Shunde District  Foshan City  Guangdong Province PRC
Institution:YE Qiaoguo,YANG Yu,HE Jing,et al Chencun Hospital of Shunde District,Foshan City,Guangdong Province 528300 PRC
Abstract:Objective To study the application of microcolumn gel technique in cross matching test and the detection of irregular antibodies. Methods Using microcolumn gel technique in cross matching test,the irregular antibodies would be screened and identified in the positive results of cross matching,and then,cross matching again with the donor whose irregular antibody corresponding antigen was negative. Results In 1051 patients,the positive results of cross matching were found in 27 cases.The irregular antibodies w...
Keywords:Microcolumn gel technique  Cross matching  Irregular antibody  Screening RBC  Panel cells  
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