| 槲皮素联合顺铂诱导宫颈HeLa细胞凋亡的实验研究 |
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| 引用本文： | 韩立薇,罗喜平,赵秋生,宋悦.槲皮素联合顺铂诱导宫颈HeLa细胞凋亡的实验研究[J].现代医院,2011,11(11):15-17. |
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| 作者姓名： | 韩立薇 罗喜平 赵秋生 宋悦 |
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| 作者单位： | 1. 广东省妇幼保健院,广东广州,510010
2. 广州中医药大学第三附属医院,广东广州,510240 |
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| 基金项目： | 广东省中医药局科研项目，广东省科技厅科技计划项目立项 |
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| 摘 要： | 目的探讨槲皮素对HeLa细胞增殖和凋亡的影响以及联合顺铂的化疗效应。方法分别用不同浓度槲皮素、顺铂及不同浓度槲皮素联合顺铂处理HeLa细胞24 h;采用MTT法检测药物对HeLa细胞增殖的影响;流式细胞仪和Hochest 33258检测药物作用后的细胞凋亡情况;荧光显微镜观察用PI荧光标记的细胞核形态学变化。结果槲皮素能抑制HeLa细胞增殖,且呈剂量依赖性;槲皮素联合顺铂与相同浓度槲皮素单用及顺铂单用处理HeLa细胞相比,前者对HeLa细胞的抑制作用更显著(p<0.01);不同浓度槲皮素与顺铂联合处理HeLa细胞与槲皮素单用及顺铂单用相比,前者显著提高了HeLa细胞的凋亡率(p<0.01)。结论槲皮素联合顺铂比槲皮素单用及顺铂单用对HeLa细胞的抑制作用更显著,因而产生更强的抗肿瘤效果。
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| 关 键 词： | 槲皮素 HeLa细胞 顺铂 宫颈癌 细胞增殖 凋亡 |
THE INFLUENCE OF QUERCETIN COMBINED WITH CISPLATIN ON THE APOPTOSIS OF CERVICAL HELA CELLS |
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| HAN Liwei,LUO Xiping,ZHAO Qiusheng,et al Guangdong Provincial Maternity , Children Healthcare Institute,Guangzhou City PRC.THE INFLUENCE OF QUERCETIN COMBINED WITH CISPLATIN ON THE APOPTOSIS OF CERVICAL HELA CELLS[J].Modern Hospital,2011,11(11):15-17. |
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| Authors: | HAN Liwei LUO Xiping ZHAO Qiusheng Guangdong Provincial Maternity Children Healthcare Institute Guangzhou City PRC |
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| Institution: | HAN Liwei,LUO Xiping,ZHAO Qiusheng,et al Guangdong Provincial Maternity and Children Healthcare Institute,Guangzhou City 510010 PRC |
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| Abstract: | Objective To discuss the influence of Quercetin on the proliferation and apoptosis of cervical cancer HeLa cells and the effects of its chemotherapy combined with Cisplatin. Methods HeLa cells were incubated for 24 h with Quercetin or Cisplatin at different consentrations respectively,or with Quercetin+Cisplatin.The influence of the proliferation of HeLa cells were evaluated by MTT assay.The rates of cell apoptosis were detected by Flow cytometry and Hochest 33258,and the nucleolus morphologic changes were ... |
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| Keywords: | Quercetin HeLa cells Cisplatin Cervical cancer Cell proliferation Apoptosis |
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