| 以HIV-1Vif与人APOBEC3G为靶点的抗HIV-1药物的研究方法 |
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| 引用本文： | 乔新华,张文俊,李泽琳,曾毅. 以HIV-1Vif与人APOBEC3G为靶点的抗HIV-1药物的研究方法[J]. 中国中药杂志, 2011, 36(6): 806-809 |
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| 作者姓名： | 乔新华 张文俊 李泽琳 曾毅 |
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| 作者单位： | 北京工业大学,生命学院,北京,100124 |
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| 基金项目： | 国家重点基础研究发展计划(973)项目(2009CB930203) |
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| 摘 要： | 人载脂蛋白B mRNA编辑酶催化多肽样蛋白3G(apolipoprotein B mRNA-editing enzyme catalytic polypeptide 3 protein G,APOBEC3G)是宿主的抗HIV-1(human immunodeficiency virus type Ⅰ)因子,而HIV-1辅助蛋白--病毒感染因子Vif(viral infectivity factor)可通过介导蛋白酶体途径降解APOBEC3G,因此针对APOBEC3G及HIV-1Vif进行抑制剂设计已经成为抗HIV-1药物研究新的方向之一,相应用于研究Vif-APOBEC3G相互作用的方法也越来越多,如免疫印迹、免疫杂交、脉冲追踪试验、生物发光共振能量转移检测、BIAcore检测等.作者将目前用于以Vif-APOBEC3G为靶点的药物的筛选及作用机制的研究方法进行了综述,为基于此的研究提供了策略.
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| 关 键 词： | APOBEC3G HIV-1 Vif 抗HIV-1抑制剂 |
| 收稿时间： | 2010-11-04 |
Research methods of antiHIV-1 inhibitors targeting at Vif -APOBEC3G axis |
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| QIAO Xinhu,ZHANG Wenjun,LI Zelin and ZENG Yi. Research methods of antiHIV-1 inhibitors targeting at Vif -APOBEC3G axis[J]. China Journal of Chinese Materia Medica, 2011, 36(6): 806-809 |
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| Authors: | QIAO Xinhu ZHANG Wenjun LI Zelin ZENG Yi |
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| Affiliation: | College of Life Science, Beijing University of Technology, Beijing 100124,China;College of Life Science, Beijing University of Technology, Beijing 100124,China;College of Life Science, Beijing University of Technology, Beijing 100124,China;College of Life Science, Beijing University of Technology, Beijing 100124,China |
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| Abstract: | The mammalian APOBEC3G protein( apolipoprotein B mRNA-editing enzyme catalytic polypeptide 3 protein G,APOBEC3G) is an important component of the cellular innate immune response to retroviral infection.APOBEC3G can extinguish HIV-1(human immunodeficiency virus type 1) infectivity by its incorporation into virus particles and subsequent cytosine deaminase activity to block replication of HIV-1.HIV-1 Vif (viral infectivity factor)suppresses various APOBEC3 proteins through a common mechanism which induces the degradation of target proteins. Therefore, the interrelation of Vif-APOBEC3G has been extensively studied, which represents attractive targets for the development of novel inhibitors.We summarize the papers in which the detection technique and methords have been developed to assay the anti-HIV activity and its mechanism, such as western-blotting, co-immunoprecipitation, pulse-chase experiments,bioluminescence resonance energy transfer, biomolecular interaction analysis.This review is towards developing therapeutics aimed at the Vif -APOBEC3G axis. |
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| Keywords: | APOBEC3G HIV-1Vif antiviral inhibitors |
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