HspB1在新疆哈萨克族食管癌组织中的表达

目的:寻找与新疆哈萨克族食管癌发生、发展密切相关的基因,为早期诊断和治疗提供依据.方法:利用二维凝胶电泳(2-DE)和基质辅助激光解吸电离飞行时间质谱(MALDI-TOF-MS)技术联用检测出新疆哈萨克族食管癌癌组织与远端正常组织的差异表达蛋白.对其中的HspB1基因进行RT-PCR验证.结果:2-DE结果显示新疆哈萨克族食管癌患者癌组织和远端正常组织在蛋白质水平有显著增高,而其mRNA表达在癌组织中为2.18±3.98,正常组织为3.06±4.69,并无显著升高(P>0.05),且与浸润深度、临床分期、分化程度无关(P>0.05),但HspB1的表达量在浸润深度组,T1-T2期T>N为7/11(63.6%),而T3-T4期T>N仅为14/37(37.8%);在分化程度组,低分化期T>N为5/9(55.6%),中高分化期T>N仅为16/39(41%).随着浸润深度和分化程度的逐步加深,由T>N向T

Expression of HspB1 in esophageal cancer in Xinjiang Kazakh patients

AIM:To obtain differentially expressed proteins in esophageal cancer in Xinjiang Kazakh patients by using a comparative proteomic method to provide clues to early diagnosis and therapy of this malignancy.METHODS:Two-dimensional gel electrophoresis(2-DE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF-MS) were utilized for the identification of proteins differentially expressed between cancer and adjacent non-cancerous tissue.The differential expression of HspB1 was further veri ed by RT-PCR.RESULTS:Overexpression of HspB1 protein was noted in esophageal cancer;however,its mRNA expression showed no significant difference between cancer tissue and normal tissue(2.18 ± 3.98 vs 3.06 ± 4.69,P > 0.05).HspB1 mRNA expression had no correlation with depth of invasion,clinical stage,or degree of differentiation(all P > 0.05).The percentage of samples showing higher HspB1 expression in esophageal cancer than in normal tissue,in patients with T3 to T4 cancer than in those with T1 to T2 cancer,and in those with high-differentiation cancer than in those with low-di erentiation cancer.CONCLUSION:Our finding suggests that HspB1 expression may be regulated by several different mechanisms during the infiltration and di erentiation of esophageal cancer.