HLA-DRB1*09/12/15与广西HBV相关性肝病的关系

目的:探讨HLA-DRB1*09/12/15等位基因频率与广西乙型肝炎病毒(hepatitis B virus,HBV)相关性肝病病情的关系.方法:应用聚合酶链反应-序列特异性引物(polymerase chain reaction-sequence specific primer,PCR-SSP)技术对64例慢性无症状HBV携带者(asymptomatic chronic hepatitis B,ASC)组、80例慢性乙型肝炎(chronic hepatitis B,CHB)组、65例乙肝肝硬化(liver cirrhosis,LC)组、56例乙肝肝细胞癌(hepatocellular carcinoma,HCC)组以及75例正常对照(NC)组的HLA-DRB1*09/12/15等位基因进行检测,分析其表达频率与慢乙肝病情的相关性.结果:乙肝肝硬化组HLA-DRB1*15等位基因的携带率(10.8%),较NC组(34.7%)、ASC组(39.1%)、HCC组(33.9%)、CHB组(33.8%)明显减低,差异具有统计学意义(P<0.01),其余各组两两比较均无统计学差异;HLA-DRB1*09/12在各组间的分布均无统计学差异.结论:HLA-DRB1*15等位基因携带者感染HBV后可能降低乙肝肝硬化发生的风险,HLA-DRB1*15可能是广西乙肝肝硬化的抵抗基因.

Association between HLADRB1* 09/12/15 alleles and HBV-related hepatic diseases

AIM:To assess the relationship between leukocyte antigen(HLA)-DRB1 alleles and HBVrelated hepatic diseases in Guangxi.METHODS:Polymerase chain reaction-sequence specific primer(PCR-SSP) method was used to determine the frequencies of HLA-DRB1* 09/12/15 alleles in 64 patients with asymptomatic chronic hepatitis B(ASC),80 patients with chronic hepatitis B(CHB),65 patients with liver cirrhosis(LC),56 patients with hepatocellular carcinoma(HCC),and 75 healthy controls from Guangxi Province,China.RESULTS:The frequency of the HLA-DRB1* 15 allele differed significantly among the five groups of subjects(P < 0.05),while the frequencies of the other alleles showed no significant differences(all P > 0.05).The frequency of the HLA-DRB1* 15 allele in patients with LC(10.8%) was significantly lower than those in patients with NC(34.7%),ASC(39.1%),HCC(33.9%),or CHB(33.8%)(all P< 0.01),but showed no signif icant difference between patients in the other four groups(all P > 0.05).CONCLUSION:The lack of the expression of HLA-DRB1*15 gene might associate with a higher risk for the development of LC in patients with HBV infection.