| 地塞米松、齐留通对哮喘大鼠5-脂氧合酶的调节 |
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| 引用本文： | 宋俊峰,路智红. 地塞米松、齐留通对哮喘大鼠5-脂氧合酶的调节[J]. 中国现代医生, 2012, 50(19): 7-8 |
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| 作者姓名： | 宋俊峰 路智红 |
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| 作者单位： | 1. 杭州市第一人民医院儿科,浙江杭州,310006
2. 浙江大学医学院附属儿童医院内科,浙江杭州,310003 |
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| 摘 要： | 目的探讨哮喘大鼠中地塞米松、齐留通与5一脂氧合酶（5一LOX）基因、蛋白表达的关系。方法制备卵蛋白致敏哮喘大鼠模型;通过原位杂交、免疫组化等方法,观察地塞米松、齐留通对模型鼠5-LOX基因、蛋白表达的影响。结果哮喘组、地塞米松组肺组织中5-LOXmRNA、蛋白表达显著高于正常组、齐留通组（P〈0．05）;齐留通＋地塞米松组5-LOXmRNA表达显著低于齐留通组（P〈0．05）。结论糖皮质激素不能抑制5-LOX基因表达、蛋白合成;齐留通可抑制5-LOX基因表达、蛋白合成;糖皮质激素、齐留通联用较齐留通单用具有更强的抑制5-LOX作用。
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| 关 键 词： | 哮喘 花生四烯酸5一脂氧合酶 脂氧合酶抑制剂 地塞米松 大鼠 |
Regulation of dexamethasone,zileuton on 5-lipoxygenase expression in the lungs of murine model of asthma |
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| SONG Junfeng , LU Zhihong. Regulation of dexamethasone,zileuton on 5-lipoxygenase expression in the lungs of murine model of asthma[J]. Journal China Modern Doctor, 2012, 50(19): 7-8 |
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| Authors: | SONG Junfeng LU Zhihong |
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| Affiliation: | 1.The Pediatric Department of the First People’s Hospital of Hangzhou in Zhejiang Province,Hangzhou 310006,China;2.Department of Internal Medicine,the Children’s Hospital,Zhejiang University School of Medicine,Hangzhou 310003,China |
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| Abstract: | Objective To investigate the effect of dexamethasone,zileuton on 5-lipoxygenase(5-LOX) expression in the lungs in murine model of asthma.Methods The asthmatic model was induced by being sensitized and challenged with ovalbumin.By morphology examination,immunohistochemistry and in situ hybridization,we observed the regulation of dexamethasone,zileuton on 5-LOX mRNA and protein expression.Results 5-LOX mRNA and protein expression in asthma,dexamethasone group increased than control and zileuton group.Expression of 5-LOX mRNA in zileuton group was higher than dexamethasone plus zileuton group.Conclusion Glucocorticoids could not inhibit 5-LOX gene and protein expression.5-LOX gene and protein expression were inhibited by zileuton.Compared with zileuton alone,glucocorticoids plus zileuton results in stronger effect on 5-LOX inhibition. |
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| Keywords: | Asthma Arachidonate 5-lipoxygenase Lipoxygenase inhibitors Dexamethasone Rats |
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