大鼠心力衰竭模型的构建与鉴定

目的比较主动脉弓缩窄和腹主动脉缩窄复制心力衰竭衰模型的异同,探索快速有效的心衰动物模型。方法将大鼠分为主动脉缩窄手术组,腹主动脉缩窄手术组和对照组(C组)。主动脉缩窄手术组实施颈部手术,在主动脉弓处缩窄动脉直径;腹主动脉缩窄手术组实施腹部手术,在腹主动脉处缩窄动脉直径;C组实施颈部手术但不实施动脉缩窄手术。各组实验动物均正常喂养4～6周后进行心脏的超声检测和心脏血流动力学检测。结果心脏超声结果显示:主动脉弓缩窄手术组左心室壁厚度和左心室腔内径在术后4周明显高于正常组;而腹主动脉缩窄手术组左心室壁厚度和左心室腔内径在术后4周较正常组没有明显增加。术后6周,腹主动脉缩窄手术组左心室壁厚度和左心室腔内径都明显增加,而主动脉弓缩窄手术组左心室壁厚度没有明显改变,左心室腔内经明显增加。血流动力学指标显示:主动脉弓缩窄手术组LVESP、LVEDP、LVDP、±dp/dtmax都明显低于腹主动脉缩窄手术组。结论主动脉弓缩窄手术复制心肌肥大导致心功能衰竭模型效果明显快于腹主动脉缩窄手术复制的心肌肥大导致心功能衰竭模型。

Establishment and Identification of Rat Models of Heart Failure

Objective To compare the difference of two heart failure models established by constriction of aortic arch and abdominal aorta,and to explore an effective method to establish an animal model of heart failure.Methods Ninety healthy adult male Wistar rats were randomly divided into three groups.The rats in the group 1 had cervical operation to contract the aortic arch,the rats in the group 2 were operated on abdomen to contract the abdominal aorta,and the rats in the group C had surgery but no aorta constriction.All the rats were fed for 4 or 6 weeks.The heart function of the rats was detected with ultrasound and heart intubation.Results At 4 weeks after constriction,ultrasonic results showed a significant increase of the left ventricular wall thickness and internal diameter of the left ventriclular cavity in the aortic arch constriction group,and not significantly increased in the abdominal aorta constriction group,compared with those of the control group.At 6 weeks after constriction,both the left ventriclular wall thickness and internal diameter of the left ventricle were significantly increased in the abdominal aorta constriction group,while only the internal diameter of the left ventricle was significantly increased and without change of the left ventriclular wall thickmess in the aortic arch constriction group.Hemodynamic detection showed that LVESP,LVEDP,LVDP and ±dp/dtmax in the aortic arch constriction group were significantly lower than those of the abdominal aorta constriction group at 6 weeks after contraction.Conclusion The progression of heart failure caused by aortic arch constriction in the rats occurs obviously faster than that developed after abdominal aorta constricction.