左心辅助对急性左心衰犬心肌c-fos mRNA的影响

目的：探讨自制气动左心辅助泵对辅助急性左心衰实验犬后c—fos mRNA表达的影响。方法：20只实验犬随机分为3组：辅助组（A组，n=9）、心衰组（B组，n=9）和对照组（C组，n=2）。A组：急性左心衰后行左心辅助循环；B组：心衰形成后不进行左心辅助；C组：开胸后未行处理。于术后6h将动物处死，用RT—PCR方法检测心肌组织c—fosmRNA的表达。结果：对照组无c—fosmRNA表达，辅助组心肌组织有少量c—fos mRNA表达，心衰组心肌组织c—fos mRNA表达较辅助组显著增加（P〈0．01）。结论：自制气动左心辅助泵抑制了心肌缺血后c—fos mRNA的表达，保护心肌细胞并抑制了继发性损害的发生，是左心辅助改善心衰可能的作用机理。

Effects of left ventricular device on c-fos mRNA in myocardial cell of canine with acute left ventricular failure

Objective:To study the effects of domestic pneumatic left ventricular assist pump on c-fos gene expression in myocardial cell of canines with acute left ventricular failure.Methods: 20 healthy dogs were divided at random into three groups: assistant group(group A,n=9),heart failure group(group B,n=9) and control group(group C,n=2).Group A received left ventricular assistance after onset of acute left ventricular failure,group B did not receive left ventricular assistance after onset of acute left ventricular failure and group C was only performed a sterno-operation without acute left ventricular failure.After operation for six hours,the dogs were killed and the levels of C-fos mRNA were detected by RT-PCR.Results: There was no evidence of c-fos mRNA expression in group C.C-fos expression increased markedly in group B and reduced in group A compared with that in group B(P<0.01).Conclusion: The domestic pneumatic left ventricular assisst device can inhibit c-fos expression after acute left ventricular failure and protect myocardial cells from secondary injury,which may be one of mechanisms that left ventricular assisst device protect function of left ventricular.