反射缺乏型干眼动物模型的研究

目的制作反射缺乏所致于眼动物模型并探讨反射缺乏在干眼发病中的作用。方法健康新西兰大白兔10只，左眼作为角膜瓣组，剪除第三眼睑及部分哈氏腺后用负压环压迫眼球，旋转式角膜板层刀做基底在上方的角膜板层切开，掀开角膜瓣暴露10s后复位；有眼作为对照组，剪除第三限睑及部分哈氏腺后仅用板层刀及负压环压迫眼球而不作角膜板层切开。分别于造模前及造模后第3、5、7、11、14、21、30d行角膜荧光素染色、SchirmerⅠ试验。在术后14、21、30d分批处死实验兔，取泪腺及角膜行光学显微镜检查。结果与造模前比较，角膜瓣组造模后角膜染色着色程度明显加重（P〈0．05）；造模后14d内，角膜瓣组与对照组的基础SchirmerⅠ试验值的差异无统计学意义（P〉0．05），14d后造模组ScbirmerⅠ试验值较对照组显著减小（P〈0．05）；角膜瓣组及对照组泪腺光镜检查结果差异无统计学意义（P〉0．05），但角膜瓣组上皮下出现较多淋巴细胞和朗格罕斯细胞浸润。结论通过第三眼睑切除联合板层角膜切开制作的反射缺乏型干眼动物模型具有手术操作简便，症状稳定，干眼持续时间较长，对泪腺干扰小，为一种较好的反射缺乏型干眼动物模型。

Establishment of an animal model of dry eye due to lack of reflection

Objective This study was to establish an animal model of dry eye due to reflection lack. Methods Ten healthy New Zealand white rabbits were used in this study. The dry eye model was created in 10 left eyes of rabbits by lamellar corneal incision for 10 seconds following to the erection of the third eyelid gland after Harrington oppression eyeball with suction ring（ corneal flap group） ,and only the third eyelid gland was removed after Harrington oppression eyeball with suction ring in 10 right eyes of rabbits（ control group）. Luciferase corneal staining and Sehirmer Ⅰ test were performed prior to operation and 3,5, 7,11,14,21,30 days after operation respectively. The rabbits were sacrificed in 14,21,30 days after operation and the lacrimal gland and cornea specimans were collected for the bistopathological examination under the light microscope. Results In the control group, the corneal fluorescence staining score was significantly elevated in 3,7 and 11 days after operation in comparison to before operation （ P 〈 0, 01 ） , and score in corneal flap group was obviously decreased in various time points after operation compared with before operation（ P 〈 0.05 - 0.01 ）. The corneal stain was significantly higher in corneal flap group than control group in the different time points （P 〈 0.05 -0.01 ）. Fourteen days later, the tear level in corneal flap group was significantly less than the control group（P 〈 0.05）. In the corneal flap group, the Schirmer I test value was significantly reduced after 14 days （P 〈 0. 05 ）. The obvious infiltration of lymphatic Langhan＇ s cells and inflammatory cells was found under the corneal epithalium in corneal flap group. No significant difference in the morphology of lacrimal gland and corneal tissue under the light microscope. Conclusion The combination procedure of the third eyelid and lamellar corneal incision can successfully induce dry eye due to reflection lack. It is an ideal animal model for the study of dry eye.