缺氧促使鼠气道平滑肌细胞丝裂原活化蛋白酶表达

目的 探讨缺氧情况下鼠气道平滑肌细胞丝裂原活化蛋白激酶（mitogen activated protein kinase,MApK）的变化.方法 雄性Wistar大鼠采用缺氧方法,用随机数字表法随机分为正常对照组、缺氧4周组,每组6只.按实验要求完成缺氧时间,12 h内留取肺叶组织,行免疫组织化学.结果 免疫组织化学染色显示,对照组大鼠肺小动脉内膜有散在免疫阳性染色,低氧组大鼠肺气道内膜则可见明显的免疫阳性染色.经灰度扫描,低氧组大鼠肺气道壁MAPK免疫染色阳性强度较对照组明显增强（P＜0.01）.结论 MAPK在缺氧性肺病引起的气道平滑肌细胞增生中可能起着重要作用.

Hypoxia prompt protein expression of mitogen activated protein kinase in rat airway smooth muscle

[Objective] To explor to change of the protein expression of Mitogen activated protein kinase in rat airway smooth muscle. [Method] 12 wistar rat were divided random into normoxia group and hypoxia group, in four weeks mitogen activated protein kinase in rat airway smooth muscle were detected by immunchemistry. [Result] Mitogen activated protein kinase in rat airway smooth muscle of normoxia group was weakly stained by immunocytochemistry, whereas the hypoxia group was strongly stained (P <0.01). [Conclusions] The results suggested hypoxia prompt the protein expression of mitogen activated protein kinase in rat airway smooth muscle, which played an important role in rat airway structureal remodeling.